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EPLIN Monoclonal antibody
EPLIN Monoclonal Antibody for WB, IHC, IF/ICC, ELISA
Host / Isotype
Mouse / IgG1
Reactivity
human, rat and More (1)
Applications
WB, IHC, IF/ICC, ELISA and More (2)
Conjugate
Unconjugated
CloneNo.
6E6E6
验证数据展示
经过测试的应用
| Positive WB detected in | HeLa cells, A431 cells, COLO 320 cells, HEK-293 cells, human brain tissue, HaCaT cells, SCaBER cells, MDA-MB-231 cells, SK-BR-3 cells, HT-29 cells, pig colon tissue, rat colon tissue, HSC-T6 cells, ROS1728 cells |
| Positive IHC detected in | human heart tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | Hela cells |
Planning an IF experiment? We recommend our CoraLite® Plus 488 and CoraLite®594 conjugated versions of this antibody.
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:10000 |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
66071-1-Ig targets EPLIN in WB, IHC, IF/ICC, CoIP, ELISA applications and shows reactivity with human, rat samples.
| Tested Applications | WB, IHC, IF/ICC, ELISA Application Description |
| Cited Applications | WB, IHC, IF, CoIP |
| Tested Reactivity | human, rat |
| Cited Reactivity | human, mouse |
| Immunogen | EPLIN fusion protein Ag13288 种属同源性预测 |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Full Name | LIM domain and actin binding 1 |
| Synonyms | LIMA1, SREBP3, PP624, LIM domain and actin-binding protein 1, LIM domain and actin binding 1 |
| Calculated Molecular Weight | 85 kDa |
| Observed Molecular Weight | 90 kDa, 110 kDa |
| GenBank Accession Number | BC001247 |
| Gene Symbol | EPLIN |
| Gene ID (NCBI) | 51474 |
| RRID | AB_11043341 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | Q9UHB6 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
EPLIN is a cytoskeletal protein that is preferentially expressed in epithelial cells and is implicated in regulation of cytoskeletal dynamics and cytokinesis. In human, there are two known isoforms, EPLIN-a and -b, generated by alternative promoter usage from a single gene. EPLIN-a expression is often down-regulated in cancerous cells and tissues. This antibody recognizes both of isoforms of EPLN.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for EPLIN antibody 66071-1-Ig | Download protocol |
| IHC protocol for EPLIN antibody 66071-1-Ig | Download protocol |
| IF protocol for EPLIN antibody 66071-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
