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  • Featured Product
  • KD/KO Validated

EPLIN Monoclonal antibody, PBS Only

EPLIN Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, rat

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

6E6E6

Cat no : 66071-1-PBS

Print datasheet

Synonyms

LIMA1, SREBP3, PP624, LIM domain and actin-binding protein 1, LIM domain and actin binding 1

验证数据展示

  • WB analysis of HEK-293 using 66071-1-Ig (same clone as 66071-1-PBS)

    WB analysis of HEK-293 using 66071-1-Ig (same clone as 66071-1-PBS)

    WB result of EPLIN antibody (66071-1-Ig, 1:2000) with si-Control and si-EPLIN transfected HEK293 cells. This data was developed using the same antibody clone with 66071-1-PBS in a different storage buffer formulation.

  • WB analysis using 66071-1-Ig (same clone as 66071-1-PBS)

    WB analysis using 66071-1-Ig (same clone as 66071-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66071-1-Ig (EPLIN antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66071-1-PBS in a different storage buffer formulation.

  • IHC staining of human heart using 66071-1-Ig (same clone as 66071-1-PBS)

    IHC staining of human heart using 66071-1-Ig (same clone as 66071-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human heart using 66071-1-Ig(EPLIN antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 66071-1-PBS in a different storage buffer formulation.

  • IHC staining of human heart using 66071-1-Ig (same clone as 66071-1-PBS)

    IHC staining of human heart using 66071-1-Ig (same clone as 66071-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human heart using 66071-1-Ig(EPLIN antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 66071-1-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 66071-1-Ig (same clone as 66071-1-PBS)

    IF Staining of HeLa using 66071-1-Ig (same clone as 66071-1-PBS)

    Immunofluorescent analysis of Hela cells using 66071-1-Ig (EPLIN antibody) at dilution of 1:50 and Rhodamine-Goat anti-Mouse IgG. This data was developed using the same antibody clone with 66071-1-PBS in a different storage buffer formulation.

  • WB analysis using 66071-1-Ig (same clone as 66071-1-PBS)

    WB analysis using 66071-1-Ig (same clone as 66071-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66071-1-Ig (EPLIN antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66071-1-PBS in a different storage buffer formulation.

产品信息

66071-1-PBS targets EPLIN in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, rat samples.

Tested Applications WB, IHC, IF/ICC, Indirect ELISA Application Description
Tested Reactivity human, rat
Immunogen EPLIN fusion protein Ag13288 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name LIM domain and actin binding 1
Synonyms LIMA1, SREBP3, PP624, LIM domain and actin-binding protein 1, LIM domain and actin binding 1
Calculated Molecular Weight 85 kDa
Observed Molecular Weight 90 kDa, 110 kDa
GenBank Accession NumberBC001247
Gene Symbol EPLIN
Gene ID (NCBI) 51474
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ9UHB6
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

EPLIN is a cytoskeletal protein that is preferentially expressed in epithelial cells and is implicated in regulation of cytoskeletal dynamics and cytokinesis. In human, there are two known isoforms, EPLIN-a and -b, generated by alternative promoter usage from a single gene. EPLIN-a expression is often down-regulated in cancerous cells and tissues. This antibody recognizes both of isoforms of EPLN.