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Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

验证数据展示

  • WB analysis using 84564-4-RR (same clone as 84564-4-PBS)

    WB analysis using 84564-4-RR (same clone as 84564-4-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 84564-4-RR (ER antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84564-4-PBS in a different storage buffer formulation.

  • IHC staining of human ovary cancer using 84564-4-RR (same clone as 84564-4-PBS)

    IHC staining of human ovary cancer using 84564-4-RR (same clone as 84564-4-PBS)

    Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 84564-4-RR (ER antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84564-4-PBS in a different storage buffer formulation.

  • IF Staining of MCF-7 using 84564-4-RR (same clone as 84564-4-PBS)

    IF Staining of MCF-7 using 84564-4-RR (same clone as 84564-4-PBS)

    Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using ER antibody (84564-4-RR, Clone: 241825D5 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 84564-4-PBS in a different storage buffer formulation.

ER Recombinant antibody, PBS Only

ER Uni-rAbTM Recombinant Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 84564-4-PBS

产品说明书

CloneNo. 241825D5

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, IF/ICC, Indirect ELISA

别名

ESR1, ESR, ERα, ER-alpha, Era

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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产品信息

84564-4-PBS targets ER in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, IF/ICC, Indirect ELISA Application Description
经测试反应性 human
免疫原 Recombinant protein 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 estrogen receptor 1
别名 ESR1, ESR, ERα, ER-alpha, Era
计算分子量 595 aa, 66 kDa
观测分子量66 kDa
GenBank蛋白编号BC128573
基因名称 ER
Gene ID (NCBI) 2099
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purfication
UNIPROT IDP03372
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

The estrogen receptor (ESR, ER) is a ligand-activated transcription factor composed of several domains important for hormone binding, DNA binding, and activation of transcription. ESR1, also known as ESR or NR3A1, belongs to the nuclear hormone receptor family and NR3 subfamily. It is a nuclear hormone receptor. The steroid hormones and their receptors are involved in the regulation of eukaryotic gene expression and affect cellular proliferation and differentiation in target tissues. ESR1 can activate the transcriptional activity of TFF1.[PMID: 11731608,10970861]