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验证数据展示

  • WB analysis using 84564-4-RR

    WB analysis using 84564-4-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84564-4-RR (ER antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IHC staining of human ovary cancer using 84564-4-RR

    IHC staining of human ovary cancer using 84564-4-RR

    Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 84564-4-RR (ER antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

ER Recombinant antibody

ER Recombinant Antibody for WB, IHC, ELISA
Cat No. 84564-4-RR

Print datasheet

CloneNo. 241825D5

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Synonyms

ESR1, ESR, ERα, ER-alpha, Era

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size: 

-/ -

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经过测试的应用

Positive WB detected inMCF-7 cells, T-47D cells
Positive IHC detected inhuman ovary cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

84564-4-RR targets ER in WB, IHC, ELISA applications and shows reactivity with human samples.

Tested Applications WB, IHC, ELISA Application Description
Tested Reactivity human
Immunogen Recombinant protein 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name estrogen receptor 1
Synonyms ESR1, ESR, ERα, ER-alpha, Era
Calculated Molecular Weight 595 aa, 66 kDa
Observed Molecular Weight66 kDa
GenBank Accession NumberBC128573
Gene Symbol ER
Gene ID (NCBI) 2099
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDP03372
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

The estrogen receptor (ESR, ER) is a ligand-activated transcription factor composed of several domains important for hormone binding, DNA binding, and activation of transcription. ESR1, also known as ESR or NR3A1, belongs to the nuclear hormone receptor family and NR3 subfamily. It is a nuclear hormone receptor. The steroid hormones and their receptors are involved in the regulation of eukaryotic gene expression and affect cellular proliferation and differentiation in target tissues. ESR1 can activate the transcriptional activity of TFF1.[PMID: 11731608,10970861]

实验方案

Product Specific Protocols
WB protocol for ER antibody 84564-4-RRDownload protocol
IHC protocol for ER antibody 84564-4-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols