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验证数据展示

  • WB analysis using 84564-4-RR

    WB analysis using 84564-4-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84564-4-RR (ER antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IHC staining of human ovary cancer using 84564-4-RR

    IHC staining of human ovary cancer using 84564-4-RR

    Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 84564-4-RR (ER antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of MCF-7 using 84564-4-RR

    IF Staining of MCF-7 using 84564-4-RR

    Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using ER antibody (84564-4-RR, Clone: 241825D5 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

ER Recombinant antibody

ER Uni-rAbTM Recombinant Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 84564-4-RR

产品说明书

CloneNo. 241825D5

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, IF/ICC, ELISA

别名

ESR1, ESR, ERα, ER-alpha, Era

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inMCF-7 cells, T-47D cells
Positive IHC detected inhuman ovary cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inMCF-7 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)/ICCIF/ICC : 1:250-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

84564-4-RR targets ER in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
经测试反应性 human
免疫原 Recombinant protein 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 estrogen receptor 1
别名 ESR1, ESR, ERα, ER-alpha, Era
计算分子量 595 aa, 66 kDa
观测分子量66 kDa
GenBank蛋白编号BC128573
基因名称 ER
Gene ID (NCBI) 2099
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purfication
UNIPROT IDP03372
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

The estrogen receptor (ESR, ER) is a ligand-activated transcription factor composed of several domains important for hormone binding, DNA binding, and activation of transcription. ESR1, also known as ESR or NR3A1, belongs to the nuclear hormone receptor family and NR3 subfamily. It is a nuclear hormone receptor. The steroid hormones and their receptors are involved in the regulation of eukaryotic gene expression and affect cellular proliferation and differentiation in target tissues. ESR1 can activate the transcriptional activity of TFF1.[PMID: 11731608,10970861]

实验方案

Product Specific Protocols
WB protocol for ER antibody 84564-4-RRDownload protocol
IHC protocol for ER antibody 84564-4-RRDownload protocol
IF protocol for ER antibody 84564-4-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols