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  • Featured Product
  • KD/KO Validated

ERAB Monoclonal antibody, PBS Only (Capture)

ERAB Monoclonal Antibody for WB, IHC, Cytometric bead array, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

WB, IHC, Cytometric bead array, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1G5E5

Cat no : 60069-1-PBS

Print datasheet

Synonyms

HSD17B10, 3-hydroxy-2-methylbutyryl-CoA dehydrogenase, 3alpha(or 20beta)-hydroxysteroid dehydrogenase, 3-alpha-(17-beta)-hydroxysteroid dehydrogenase, 2-methyl-3-hydroxybutyryl-CoA dehydrogenase

验证数据展示

  • WB analysis of HEK-293 using 60069-1-Ig (same clone as 60069-1-PBS)

    WB analysis of HEK-293 using 60069-1-Ig (same clone as 60069-1-PBS)

    WB result of ERAB antibody (60069-1-Ig; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-ERAB transfected HEK-293 cells. This data was developed using the same antibody clone with 60069-1-PBS in a different storage buffer formulation.

  • WB analysis of ROS1728 using 60069-1-Ig (same clone as 60069-1-PBS)

    WB analysis of ROS1728 using 60069-1-Ig (same clone as 60069-1-PBS)

    ROS1728 cells were subjected to SDS PAGE followed by western blot with 60069-1-Ig (ERAB Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60069-1-PBS in a different storage buffer formulation.

  • IHC staining of human liver cancer using 60069-1-Ig (same clone as 60069-1-PBS)

    IHC staining of human liver cancer using 60069-1-Ig (same clone as 60069-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 60069-1-Ig (ERAB antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60069-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with HSD17B10 antibody included
  • Cytometric bead array standard curve of MP50654-2

    Cytometric bead array standard curve of MP50654-2

    Cytometric bead array standard curve of MP50654-2, HSD17B10 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 60069-1-PBS. Detection antibody: 60069-4-PBS. Standard:Ag1020. Range: 0.098-50 ng/mL.

Planning an IHC experiment? We recommend our IHCeasy HSD17B10 Ready-To-Use IHC Kit. HSD17B10 primary antibody included.

产品信息

60069-1-PBS targets ERAB as part of a matched antibody pair:

MP50654-2: 60069-1-PBS capture and 60069-4-PBS detection (validated in Cytometric bead array)

Unconjugated mouse monoclonal antibody pair in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Applications WB, IHC, Cytometric bead array, Indirect ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen ERAB fusion protein Ag1020 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name hydroxysteroid (17-beta) dehydrogenase 10
Synonyms HSD17B10, 3-hydroxy-2-methylbutyryl-CoA dehydrogenase, 3alpha(or 20beta)-hydroxysteroid dehydrogenase, 3-alpha-(17-beta)-hydroxysteroid dehydrogenase, 2-methyl-3-hydroxybutyryl-CoA dehydrogenase
Calculated Molecular Weight 26 kDa
Observed Molecular Weight 27 kDa
GenBank Accession NumberBC008708
Gene Symbol ERAB
Gene ID (NCBI) 3028
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDQ99714
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

HSD17B10 (3-hydroxyacyl-CoA dehydrogenase type-2) is a multifunctional mitochondrial enzyme that acts on a wide spectrum of substrates, including neuroactive steroids, alcohols, leucine, and fatty acids, with a preference for short-chain methyl-branched acyl-CoAs(PMID:15860413).It has 2 isoforms produced by alternative splicing.Defects in HSD17B10 are the cause of 2-methyl-3-hydroxybutyryl-CoA dehydrogenase deficiency (MHBD deficiency) and mental retardation syndromic X-linked type 10 (MRXS10).