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验证数据展示

  • WB analysis of HEK-293 using 60069-1-Ig

    WB analysis of HEK-293 using 60069-1-Ig

    WB result of ERAB antibody (60069-1-Ig; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-ERAB transfected HEK-293 cells.

  • WB analysis of ROS1728 using 60069-1-Ig

    WB analysis of ROS1728 using 60069-1-Ig

    ROS1728 cells were subjected to SDS PAGE followed by western blot with 60069-1-Ig (ERAB Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • IHC staining of human liver cancer using 60069-1-Ig

    IHC staining of human liver cancer using 60069-1-Ig

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 60069-1-Ig (ERAB antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with HSD17B10 antibody included
  • Featured Product
  • KD/KO验证

ERAB Monoclonal antibody

ERAB Monoclonal Antibody for WB, IHC, ELISA
Cat No. 60069-1-Ig

产品说明书

CloneNo. 1G5E5

宿主/亚型

Mouse / IgG1

种属反应性

human, mouse, rat

应用

WB, IHC, ELISA

别名

17 beta HSD 10, 17b HSD10, ABAD, CAMR, DUPXp11.22, ERAB, HADH2, HCD2, HSD17B10, MHBD, MRPP2, MRX17, MRX31, MRXS10, SCHAD, SDR5C1, Type II HADH, XH98G2

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inROS1728 cells, RAW 264.7 cells, Jurkat cells, HEK-293 cells, HeLa cells
Positive IHC detected inhuman liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy HSD17B10 Ready-To-Use IHC Kit. HSD17B10 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:1000-1:4000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

60069-1-Ig targets ERAB in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 ERAB fusion protein Ag1020 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 hydroxysteroid (17-beta) dehydrogenase 10
别名 17 beta HSD 10, 17b HSD10, ABAD, CAMR, DUPXp11.22, ERAB, HADH2, HCD2, HSD17B10, MHBD, MRPP2, MRX17, MRX31, MRXS10, SCHAD, SDR5C1, Type II HADH, XH98G2
计算分子量 26 kDa
观测分子量 27 kDa
GenBank蛋白编号BC008708
基因名称 ERAB
Gene ID (NCBI) 3028
RRIDAB_2119779
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein G purification
UNIPROT IDQ99714
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

HSD17B10 (3-hydroxyacyl-CoA dehydrogenase type-2) is a multifunctional mitochondrial enzyme that acts on a wide spectrum of substrates, including neuroactive steroids, alcohols, leucine, and fatty acids, with a preference for short-chain methyl-branched acyl-CoAs(PMID:15860413).It has 2 isoforms produced by alternative splicing.Defects in HSD17B10 are the cause of 2-methyl-3-hydroxybutyryl-CoA dehydrogenase deficiency (MHBD deficiency) and mental retardation syndromic X-linked type 10 (MRXS10).

实验方案

Product Specific Protocols
WB protocol for ERAB antibody 60069-1-IgDownload protocol
IHC protocol for ERAB antibody 60069-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols