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验证数据展示

  • WB analysis of HEK-293 using 60069-1-Ig

    WB analysis of HEK-293 using 60069-1-Ig

    WB result of ERAB antibody (60069-1-Ig; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-ERAB transfected HEK-293 cells.

  • WB analysis of ROS1728 using 60069-1-Ig

    WB analysis of ROS1728 using 60069-1-Ig

    ROS1728 cells were subjected to SDS PAGE followed by western blot with 60069-1-Ig (ERAB Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • IHC staining of human liver cancer using 60069-1-Ig

    IHC staining of human liver cancer using 60069-1-Ig

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 60069-1-Ig (ERAB antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with HSD17B10 antibody included
  • Featured Product
  • KD/KO Validated

ERAB Monoclonal antibody

ERAB Monoclonal Antibody for WB, IHC, ELISA
Cat No. 60069-1-Ig

Print datasheet

CloneNo. 1G5E5

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Synonyms

17 beta HSD 10, 17b HSD10, ABAD, CAMR, DUPXp11.22, ERAB, HADH2, HCD2, HSD17B10, MHBD, MRPP2, MRX17, MRX31, MRXS10, SCHAD, SDR5C1, Type II HADH, XH98G2

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size: 

-/ -

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经过测试的应用

Positive WB detected inROS1728 cells, RAW 264.7 cells, Jurkat cells, HEK-293 cells, HeLa cells
Positive IHC detected inhuman liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy HSD17B10 Ready-To-Use IHC Kit. HSD17B10 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:1000-1:4000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

60069-1-Ig targets ERAB in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen ERAB fusion protein Ag1020 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name hydroxysteroid (17-beta) dehydrogenase 10
Synonyms 17 beta HSD 10, 17b HSD10, ABAD, CAMR, DUPXp11.22, ERAB, HADH2, HCD2, HSD17B10, MHBD, MRPP2, MRX17, MRX31, MRXS10, SCHAD, SDR5C1, Type II HADH, XH98G2
Calculated Molecular Weight 26 kDa
Observed Molecular Weight 27 kDa
GenBank Accession NumberBC008708
Gene Symbol ERAB
Gene ID (NCBI) 3028
RRIDAB_2119779
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDQ99714
Storage Buffer PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

HSD17B10 (3-hydroxyacyl-CoA dehydrogenase type-2) is a multifunctional mitochondrial enzyme that acts on a wide spectrum of substrates, including neuroactive steroids, alcohols, leucine, and fatty acids, with a preference for short-chain methyl-branched acyl-CoAs(PMID:15860413).It has 2 isoforms produced by alternative splicing.Defects in HSD17B10 are the cause of 2-methyl-3-hydroxybutyryl-CoA dehydrogenase deficiency (MHBD deficiency) and mental retardation syndromic X-linked type 10 (MRXS10).

实验方案

Product Specific Protocols
WB protocol for ERAB antibody 60069-1-IgDownload protocol
IHC protocol for ERAB antibody 60069-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols