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Species Reactivity
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验证数据展示

  • WB analysis of HEK-293T using 29465-1-AP (same clone as 29465-1-PBS)

    WB analysis of HEK-293T using 29465-1-AP (same clone as 29465-1-PBS)

    HEK-293T cells were subjected to SDS PAGE followed by western blot with 29465-1-AP (ERAL1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 29465-1-PBS in a different storage buffer formulation.

  • IHC staining of human colon using 29465-1-AP (same clone as 29465-1-PBS)

    IHC staining of human colon using 29465-1-AP (same clone as 29465-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 29465-1-AP (ERAL1 antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 29465-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with ERAL1 antibody included
  • IHC staining of human colon using 29465-1-AP (same clone as 29465-1-PBS)

    IHC staining of human colon using 29465-1-AP (same clone as 29465-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 29465-1-AP (ERAL1 antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 29465-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with ERAL1 antibody included

ERAL1 Polyclonal antibody, PBS Only

ERAL1 Polyclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 29465-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, Indirect ELISA

别名

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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Planning an IHC experiment? We recommend our IHCeasy ERAL1 Ready-To-Use IHC Kit. ERAL1 primary antibody included.

产品信息

29465-1-PBS targets ERAL1 in WB, IHC, Indirect ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, Indirect ELISA Application Description
经测试反应性 human
免疫原 ERAL1 fusion protein Ag30720 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 Era G-protein-like 1 (E. coli)
别名
计算分子量 437 aa, 48 kDa
观测分子量 40-45 kDa
GenBank蛋白编号BC019094
基因名称 ERAL1
Gene ID (NCBI) 26284
RRIDAB_2918311
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDO75616
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

ERAL1, a homologue of Era protein in Escherichia coli, is a member of conserved GTP-binding proteins with RNA-binding activity. ERAL1 localizes to the mitochondrionmitoribosomal subunit, and binds to the 3' terminal stem loop of 12S mitochondrial rRNA and is required for proper assembly of the 28S small mitochondrial ribosomal subunit. Deletion of ERAL1 has been shown to cause mitochondrial dysfunction, growth retardation, and apoptosis.