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验证数据展示

  • WB analysis of K-562 using 14637-1-AP

    WB analysis of K-562 using 14637-1-AP

    K-562 cells were subjected to SDS PAGE followed by western blot with 14637-1-AP (ERAP2 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • IHC staining of human lung cancer using 14637-1-AP

    IHC staining of human lung cancer using 14637-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 14637-1-AP (ERAP2 antibody) at dilution of 1:50 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human lung cancer using 14637-1-AP

    IHC staining of human lung cancer using 14637-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 14637-1-AP (ERAP2 antibody) at dilution of 1:50 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of HepG2 using 14637-1-AP

    IF Staining of HepG2 using 14637-1-AP

    Immunofluorescent analysis of (-20℃ Ethanol) fixed HepG2 cells using 14637-1-AP (ERAP2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

ERAP2 Polyclonal antibody

ERAP2 Polyclonal Antibody for WB, IF, IHC, ELISA
Cat No. 14637-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IF, IHC, ELISA

别名

ERAP2, L RAP, LRAP

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inK-562 cells, A549 cells, mouse thymus tissue
Positive IHC detected inhuman lung cancer tissue, human liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

14637-1-AP targets ERAP2 in WB, IF, IHC, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IF, IHC, ELISA Application Description
经测试反应性 human, mouse
免疫原 ERAP2 fusion protein Ag6216 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 endoplasmic reticulum aminopeptidase 2
别名 ERAP2, L RAP, LRAP
计算分子量 110 kDa
观测分子量 61-65 kDa
GenBank蛋白编号BC065240
基因名称 ERAP2
Gene ID (NCBI) 64167
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ6P179
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

ERAP2(Endoplasmic reticulum aminopeptidase 2) is also named as LRAP(Leukocyte-derived arginine aminopeptidase) and belongs to the peptidase M1 family. It plays a central role in peptide trimming, a step required for the generation of most HLA class I-binding peptides. It can form heterodimers with ERAP1(PMID:15908954). It has 4 isoforms produced by alternative splicing. And defects in the expression of this gene may cause improper antigen processing, possibly leading to favor tumor escape from the immune surveillance.