• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

ERAP2 Polyclonal antibody

ERAP2 Polyclonal Antibody for WB, IF, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IF, IHC, ELISA

Conjugate

Unconjugated

Cat no : 14637-1-AP

Print datasheet

Synonyms

ERAP2, L RAP, LRAP

验证数据展示

  • WB analysis of K-562 using 14637-1-AP

    WB analysis of K-562 using 14637-1-AP

    K-562 cells were subjected to SDS PAGE followed by western blot with 14637-1-AP (ERAP2 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • IHC staining of human lung cancer using 14637-1-AP

    IHC staining of human lung cancer using 14637-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 14637-1-AP (ERAP2 antibody) at dilution of 1:50 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human lung cancer using 14637-1-AP

    IHC staining of human lung cancer using 14637-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 14637-1-AP (ERAP2 antibody) at dilution of 1:50 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of HepG2 using 14637-1-AP

    IF Staining of HepG2 using 14637-1-AP

    Immunofluorescent analysis of (-20℃ Ethanol) fixed HepG2 cells using 14637-1-AP (ERAP2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

经过测试的应用

Positive WB detected inK-562 cells, A549 cells, mouse thymus tissue
Positive IHC detected inhuman lung cancer tissue, human liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

14637-1-AP targets ERAP2 in WB, IF, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications WB, IF, IHC, ELISA Application Description
Tested Reactivity human, mouse
Immunogen ERAP2 fusion protein Ag6216 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name endoplasmic reticulum aminopeptidase 2
Synonyms ERAP2, L RAP, LRAP
Calculated Molecular Weight 110 kDa
Observed Molecular Weight 61-65 kDa
GenBank Accession NumberBC065240
Gene Symbol ERAP2
Gene ID (NCBI) 64167
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ6P179
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

ERAP2(Endoplasmic reticulum aminopeptidase 2) is also named as LRAP(Leukocyte-derived arginine aminopeptidase) and belongs to the peptidase M1 family. It plays a central role in peptide trimming, a step required for the generation of most HLA class I-binding peptides. It can form heterodimers with ERAP1(PMID:15908954). It has 4 isoforms produced by alternative splicing. And defects in the expression of this gene may cause improper antigen processing, possibly leading to favor tumor escape from the immune surveillance.