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验证数据展示

  • WB analysis using 68159-1-Ig

    WB analysis using 68159-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68159-1-Ig (ERBB2IP antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • WB analysis using 68159-1-Ig

    WB analysis using 68159-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68159-1-Ig (ERBB2IP antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • WB analysis of HeLa using 68159-1-Ig

    WB analysis of HeLa using 68159-1-Ig

    WB result of ERBB2IP antibody (68159-1-Ig; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-ERBB2IP transfected HeLa cells.

  • IF Staining of MCF-7 using 68159-1-Ig

    IF Staining of MCF-7 using 68159-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using ERBB2IP antibody (68159-1-Ig, Clone: 1A8D4 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1).

  • Featured Product
  • KD/KO Validated

ERBB2IP Monoclonal antibody

ERBB2IP Monoclonal Antibody for WB, IF/ICC, ELISA
Cat No. 68159-1-Ig

Print datasheet

CloneNo. 1A8D4

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, ELISA

Synonyms

LAP2, KIAA1225, ERBIN, Erbb2-interacting protein, Densin-180-like protein

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size: 

-/ -

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经过测试的应用

Positive WB detected inU2OS cells, HSC-T6 cells, HeLa cells, A549 cells, LNCaP cells, MCF-7 cells, HEK-293T cells, Jurkat cells, NIH/3T3 cells
Positive IF/ICC detected inMCF-7 cells
Planning an IHC experiment? We recommend our IHCeasy ERBIN Ready-To-Use IHC Kit. ERBIN primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

68159-1-Ig targets ERBB2IP in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IF/ICC, ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen ERBB2IP fusion protein Ag18514 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name erbb2 interacting protein
Synonyms LAP2, KIAA1225, ERBIN, Erbb2-interacting protein, Densin-180-like protein
Calculated Molecular Weight 1412 aa, 158 kDa
Observed Molecular Weight 150-180 kDa
GenBank Accession NumberBC115012
Gene Symbol ERBB2IP
Gene ID (NCBI) 55914
RRIDAB_3085045
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ96RT1
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

ERBB2IP is a cytoplasmic protein that contains leucine-rich repeats (LRR) and PSD95/Dlg1/zo-1 (PDZ) domain (thus named a LAP protein). ERBB2IP interacts, via its single PDZ domain, specifically with ErbB2, but not with ErbB3, ErbB4, or EGFR. It is colocalized with ErbB2 at the basolateral membranes of epithelial cells. In addition, ERBB2IP also interacts with PSD‐95, Lano and with three p120‐related catenins, eBPAG1 and the β4 subunit of the α6β4 integrin, two major components of hemidesmosomes. The molecular weight of ERBB2IP was predicted 150~180 kDa by Western Blot.(PMID: 25288731,PMID: 24252174,PMID: 19289088)

实验方案

Product Specific Protocols
WB protocol for ERBB2IP antibody 68159-1-IgDownload protocol
IF protocol for ERBB2IP antibody 68159-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols