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验证数据展示

  • WB analysis using 68159-1-Ig (same clone as 68159-1-PBS)

    WB analysis using 68159-1-Ig (same clone as 68159-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68159-1-Ig (ERBB2IP antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68159-1-PBS in a different storage buffer formulation.

  • WB analysis using 68159-1-Ig (same clone as 68159-1-PBS)

    WB analysis using 68159-1-Ig (same clone as 68159-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68159-1-Ig (ERBB2IP antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68159-1-PBS in a different storage buffer formulation.

  • WB analysis of HeLa using 68159-1-Ig (same clone as 68159-1-PBS)

    WB analysis of HeLa using 68159-1-Ig (same clone as 68159-1-PBS)

    WB result of ERBB2IP antibody (68159-1-Ig; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-ERBB2IP transfected HeLa cells. This data was developed using the same antibody clone with 68159-1-PBS in a different storage buffer formulation.

  • IF Staining of MCF-7 using 68159-1-Ig (same clone as 68159-1-PBS)

    IF Staining of MCF-7 using 68159-1-Ig (same clone as 68159-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using ERBB2IP antibody (68159-1-Ig, Clone: 1A8D4 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1). This data was developed using the same antibody clone with 68159-1-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP51110-1

    Cytometric bead array standard curve of MP51110-1

    Cytometric bead array standard curve of MP51110-1, ERBB2IP Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 68159-1-PBS. Detection antibody: 68159-2-PBS. Standard:Ag18514. Range: 0.195-100 ng/mL.

  • Featured Product
  • KD/KO验证

ERBB2IP Monoclonal antibody, PBS Only (Capture)

ERBB2IP Monoclonal Antibody for WB, IF/ICC, Cytometric bead array, Indirect ELISA
Cat No. 68159-1-PBS

产品说明书

CloneNo. 1A8D4

宿主/亚型

Mouse / IgG1

种属反应性

human, mouse, rat

应用

WB, IF/ICC, Cytometric bead array, Indirect ELISA

别名

LAP2, KIAA1225, ERBIN, Erbb2-interacting protein, Densin-180-like protein

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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Planning an IHC experiment? We recommend our IHCeasy ERBIN Ready-To-Use IHC Kit. ERBIN primary antibody included.

产品信息

68159-1-PBS targets ERBB2IP as part of a matched antibody pair:

MP51110-1: 68159-1-PBS capture and 68159-2-PBS detection (validated in Cytometric bead array)

Unconjugated mouse monoclonal antibody pair in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

经测试应用 WB, IF/ICC, Cytometric bead array, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 ERBB2IP fusion protein Ag18514 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 erbb2 interacting protein
别名 LAP2, KIAA1225, ERBIN, Erbb2-interacting protein, Densin-180-like protein
计算分子量 1412 aa, 158 kDa
观测分子量 150-180 kDa
GenBank蛋白编号BC115012
基因名称 ERBB2IP
Gene ID (NCBI) 55914
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ96RT1
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

ERBB2IP is a cytoplasmic protein that contains leucine-rich repeats (LRR) and PSD95/Dlg1/zo-1 (PDZ) domain (thus named a LAP protein). ERBB2IP interacts, via its single PDZ domain, specifically with ErbB2, but not with ErbB3, ErbB4, or EGFR. It is colocalized with ErbB2 at the basolateral membranes of epithelial cells. In addition, ERBB2IP also interacts with PSD‐95, Lano and with three p120‐related catenins, eBPAG1 and the β4 subunit of the α6β4 integrin, two major components of hemidesmosomes. The molecular weight of ERBB2IP was predicted 150~180 kDa by Western Blot.(PMID: 25288731,PMID: 24252174,PMID: 19289088)