ERCC1 Monoclonal antibody

ERCC1 Monoclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

1H4D5

Cat no : 66275-1-Ig

Print datasheet

Synonyms

COFS4, ERCC1, UV20



经过测试的应用

Positive WB detected inA431 cells
Positive IF/ICC detected inA431 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

66275-1-Ig targets ERCC1 in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Applications WB, IF/ICC, ELISA Application Description
Cited ApplicationsWB
Tested Reactivity human
Cited Reactivityhuman
Immunogen ERCC1 fusion protein Ag6417 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name excision repair cross-complementing rodent repair deficiency, complementation group 1 (includes overlapping antisense sequence)
Synonyms COFS4, ERCC1, UV20
Calculated Molecular Weight 33 kDa
Observed Molecular Weight 38 kDa
GenBank Accession NumberBC052813
Gene Symbol ERCC1
Gene ID (NCBI) 2067
RRIDAB_2881658
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDP07992
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Excision Repair Cross Complementing 1 (ERCC1) is a structure-specific endonuclease that is responsible for the 5'-incision during DNA repair. It forms a complex with ERCC11, XPF and ERCC4, which are required in both recombinatorial repair and nucleotide excision repair. It has been found that ERCC1, together with RRM1, are determinants of survival after surgical treatment of early-stage, non-small-cell lung cancer. (Ref: Simon, ER. 2005)

实验方案

Product Specific Protocols
WB protocol for ERCC1 antibody 66275-1-IgDownload protocol
IF protocol for ERCC1 antibody 66275-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Biochem Pharmacol

PMID: 31541631

Authors - Bin Zhu