ERCC1 Monoclonal antibody

ERCC1 Monoclonal Antibody for WB, IF/ICC, ELISA
Cat No. 66275-1-Ig

产品说明书

CloneNo. 1H4D5

宿主/亚型

Mouse / IgG1

种属反应性

human

应用

WB, IF/ICC, ELISA

COFS4, ERCC1, UV20

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -


经过测试的应用

Positive WB detected inA431 cells
Positive IF/ICC detected inA431 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:4000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

66275-1-Ig targets ERCC1 in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IF/ICC, ELISA Application Description
文献引用应用WB
经测试反应性 human
文献引用反应性human
免疫原 ERCC1 fusion protein Ag6417 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 excision repair cross-complementing rodent repair deficiency, complementation group 1 (includes overlapping antisense sequence)
别名 COFS4, ERCC1, UV20
计算分子量 33 kDa
观测分子量 38 kDa
GenBank蛋白编号BC052813
基因名称 ERCC1
Gene ID (NCBI) 2067
RRIDAB_2881658
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein G purification
UNIPROT IDP07992
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Excision Repair Cross Complementing 1 (ERCC1) is a structure-specific endonuclease that is responsible for the 5'-incision during DNA repair. It forms a complex with ERCC11, XPF and ERCC4, which are required in both recombinatorial repair and nucleotide excision repair. It has been found that ERCC1, together with RRM1, are determinants of survival after surgical treatment of early-stage, non-small-cell lung cancer. (Ref: Simon, ER. 2005)

实验方案

Product Specific Protocols
WB protocol for ERCC1 antibody 66275-1-IgDownload protocol
IF protocol for ERCC1 antibody 66275-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Biochem Pharmacol

PMID: 31541631

Authors - Bin Zhu
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