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ERCC1 Monoclonal antibody, PBS Only

ERCC1 Monoclonal Antibody for WB, IF/ICC, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

WB, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1H4D5

Cat no : 66275-1-PBS

Print datasheet

Synonyms

COFS4, ERCC1, UV20

验证数据展示

  • WB analysis of A431 using 66275-1-Ig (same clone as 66275-1-PBS)

    WB analysis of A431 using 66275-1-Ig (same clone as 66275-1-PBS)

    A431 cells were subjected to SDS PAGE followed by western blot with 66275-1-Ig (ERCC1 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66275-1-PBS in a different storage buffer formulation.

  • IF Staining of A431 using 66275-1-Ig (same clone as 66275-1-PBS)

    IF Staining of A431 using 66275-1-Ig (same clone as 66275-1-PBS)

    Immunofluorescent analysis of (10% Formaldehyde ) fixed A431 cells using 66275-1-Ig(ERCC1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66275-1-PBS in a different storage buffer formulation.

产品信息

66275-1-PBS targets ERCC1 in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

Tested Applications WB, IF/ICC, Indirect ELISA Application Description
Tested Reactivity human
Immunogen ERCC1 fusion protein Ag6417 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name excision repair cross-complementing rodent repair deficiency, complementation group 1 (includes overlapping antisense sequence)
Synonyms COFS4, ERCC1, UV20
Calculated Molecular Weight 33 kDa
Observed Molecular Weight 38 kDa
GenBank Accession NumberBC052813
Gene Symbol ERCC1
Gene ID (NCBI) 2067
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDP07992
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Excision Repair Cross Complementing 1 (ERCC1) is a structure-specific endonuclease that is responsible for the 5'-incision during DNA repair. It forms a complex with ERCC11, XPF and ERCC4, which are required in both recombinatorial repair and nucleotide excision repair. It has been found that ERCC1, together with RRM1, are determinants of survival after surgical treatment of early-stage, non-small-cell lung cancer. (Ref: Simon, ER. 2005)