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验证数据展示

  • WB analysis of A431 using 66275-1-Ig (same clone as 66275-1-PBS)

    WB analysis of A431 using 66275-1-Ig (same clone as 66275-1-PBS)

    A431 cells were subjected to SDS PAGE followed by western blot with 66275-1-Ig (ERCC1 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66275-1-PBS in a different storage buffer formulation.

  • IF Staining of A431 using 66275-1-Ig (same clone as 66275-1-PBS)

    IF Staining of A431 using 66275-1-Ig (same clone as 66275-1-PBS)

    Immunofluorescent analysis of (10% Formaldehyde ) fixed A431 cells using 66275-1-Ig(ERCC1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66275-1-PBS in a different storage buffer formulation.

ERCC1 Monoclonal antibody, PBS Only

ERCC1 Monoclonal Antibody for WB, IF/ICC, Indirect ELISA
Cat No. 66275-1-PBS

产品说明书

CloneNo. 1H4D5

宿主/亚型

Mouse / IgG1

种属反应性

human

应用

WB, IF/ICC, Indirect ELISA

别名

COFS4, ERCC1, UV20

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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产品信息

66275-1-PBS targets ERCC1 in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

经测试应用 WB, IF/ICC, Indirect ELISA Application Description
经测试反应性 human
免疫原 ERCC1 fusion protein Ag6417 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 excision repair cross-complementing rodent repair deficiency, complementation group 1 (includes overlapping antisense sequence)
别名 COFS4, ERCC1, UV20
计算分子量 33 kDa
观测分子量 38 kDa
GenBank蛋白编号BC052813
基因名称 ERCC1
Gene ID (NCBI) 2067
RRIDAB_2881658
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein G purification
UNIPROT IDP07992
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Excision Repair Cross Complementing 1 (ERCC1) is a structure-specific endonuclease that is responsible for the 5'-incision during DNA repair. It forms a complex with ERCC11, XPF and ERCC4, which are required in both recombinatorial repair and nucleotide excision repair. It has been found that ERCC1, together with RRM1, are determinants of survival after surgical treatment of early-stage, non-small-cell lung cancer. (Ref: Simon, ER. 2005)