• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

验证数据展示

  • WB analysis using 15688-1-AP (same clone as 15688-1-PBS)

    WB analysis using 15688-1-AP (same clone as 15688-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 15688-1-AP (ERCC6L antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15688-1-PBS in a different storage buffer formulation.

  • IHC staining of human colon cancer using 15688-1-AP (same clone as 15688-1-PBS)

    IHC staining of human colon cancer using 15688-1-AP (same clone as 15688-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 15688-1-AP (ERCC6L antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15688-1-PBS in a different storage buffer formulation.

  • IHC staining of human colon cancer using 15688-1-AP (same clone as 15688-1-PBS)

    IHC staining of human colon cancer using 15688-1-AP (same clone as 15688-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 15688-1-AP (ERCC6L antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15688-1-PBS in a different storage buffer formulation.

  • WB analysis of Jurkat using 15688-1-AP (same clone as 15688-1-PBS)

    WB analysis of Jurkat using 15688-1-AP (same clone as 15688-1-PBS)

    WB result of ERCC6L antibody (15688-1-AP; 1:6000; incubated at room temperature for 1.5 hours) with sh-Control and sh-ERCC6L transfected Jurkat cells. This data was developed using the same antibody clone with 15688-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO验证

ERCC6L Polyclonal antibody, PBS Only

ERCC6L Polyclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 15688-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, Indirect ELISA

别名

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

点击咨询售前客服

点击咨询售后客服

产品信息

15688-1-PBS targets ERCC6L in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, Indirect ELISA Application Description
经测试反应性 human, mouse
免疫原 ERCC6L fusion protein Ag8223 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 excision repair cross-complementing rodent repair deficiency, complementation group 6-like
别名
计算分子量 419 aa, 46 kDa, 141 kDa
观测分子量 180 kDa
GenBank蛋白编号BC008808
基因名称 ERCC6L
Gene ID (NCBI) 54821
RRIDAB_2098171
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ2NKX8
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

ERCC6L, also named as Tumor antigen BJ-HCC-15 or PICH, is a 1250 amino acid protein, which contains one helicase ATP-binding domain, one helicase C-terminal domain and two TPR repeats. ERCC6L localizes to kinetochores, inner centromeres and belongs to the SNF2/RAD54 helicase family. ERCC6L as a DNA helicase that acts as an essential component of the spindle assembly checkpoint. The calculated molecular weight of ERCC6L is 140 kDa, but the phosphorylated modification of molecular weight of ERCC6L is about 180kDa.