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ERK1/2 Recombinant antibody, PBS Only

ERK1/2 Uni-rAbTM Recombinant Antibody for WB, IHC, IF/ICC, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

4P8

Cat no : 83533-1-PBS

Print datasheet

Synonyms

ERK1, ERK, ERK2, ERK-1, EC:2.7.11.24

验证数据展示

  • WB analysis using 83533-1-RR (same clone as 83533-1-PBS)

    WB analysis using 83533-1-RR (same clone as 83533-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 83533-1-RR (ERK1/2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83533-1-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 83533-1-RR (same clone as 83533-1-PBS)

    IF Staining of HeLa using 83533-1-RR (same clone as 83533-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using ERK1/2 antibody (83533-1-RR, Clone: 4P8 ) at dilution of 1:300 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1). This data was developed using the same antibody clone with 83533-1-PBS in a different storage buffer formulation.

  • IHC staining of human cervical cancer using 83533-1-RR (same clone as 83533-1-PBS)

    IHC staining of human cervical cancer using 83533-1-RR (same clone as 83533-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 83533-1-RR (ERK1/2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83533-1-PBS in a different storage buffer formulation.

  • FC experiment of HepG2 using 83533-1-RR (same clone as 83533-1-PBS)

    FC experiment of HepG2 using 83533-1-RR (same clone as 83533-1-PBS)

    1x10^6 HepG2 cells were intracellularly stained with 0.25 ug ERK1/2 Recombinant antibody (83533-1-RR, Clone:4P8) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Control Antibody (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 83533-1-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 83533-1-RR

    Affinity and Kinetic Characterization of 83533-1-RR

    Biolayer interferometry (BLl) kinetic assays of 83533-1-RR against Human ERK1/2 were performed. The affinity constant is 17.8 nM.

产品信息

The immunogen of 83533-1-PBS is ERK1/2 Fusion Protein expressed in E. coli.

Tested Applications WB, IHC, IF/ICC, FC (Intra), ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen ERK1/2 fusion protein Ag20350 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name mitogen-activated protein kinase 3
Synonyms ERK1, ERK, ERK2, ERK-1, EC:2.7.11.24
Calculated Molecular Weight 43 kDa
Observed Molecular Weight 38-44 kDa
GenBank Accession NumberBC013992
Gene Symbol ERK1
Gene ID (NCBI) 5595
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDP27361
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

ERK1 and ERK2 belongs to the protein kinase superfamily. It is involved in both the initiation and regulation of meiosis, mitosis, and postmitotic functions in differentiated cells by phosphorylating a number of transcription factors such as ELK-1. ERK1/2 catalized the reaction: ATP + a protein = ADP + a phosphoprotein. It is activated by tyrosine phosphorylation in response to INS and NGF. This antibody can recognize both ERK1 and ERK2 with the molecular mass of 38-44 kDa.