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验证数据展示

  • WB analysis using 83533-1-RR

    WB analysis using 83533-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83533-1-RR (ERK1/2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IF Staining of HeLa using 83533-1-RR

    IF Staining of HeLa using 83533-1-RR

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using ERK1/2 antibody (83533-1-RR, Clone: 4P8 ) at dilution of 1:300 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1).

  • IHC staining of human cervical cancer using 83533-1-RR

    IHC staining of human cervical cancer using 83533-1-RR

    Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 83533-1-RR (ERK1/2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • FC experiment of HepG2 using 83533-1-RR

    FC experiment of HepG2 using 83533-1-RR

    1x10^6 HepG2 cells were intracellularly stained with 0.25 ug ERK1/2 Recombinant antibody (83533-1-RR, Clone:4P8) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Control Antibody (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

  • Affinity and Kinetic Characterization of 83533-1-RR

    Affinity and Kinetic Characterization of 83533-1-RR

    Biolayer interferometry (BLl) kinetic assays of 83533-1-RR against Human ERK1/2 were performed. The affinity constant is 17.8 nM.

ERK1/2 Recombinant antibody

ERK1/2 Recombinant Antibody for WB, IHC, IF/ICC, FC (Intra), ELISA
Cat No. 83533-1-RR

产品说明书

CloneNo. 4P8

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, FC (Intra), ELISA

别名

ERK1, ERK, ERK2, ERK-1, EC:2.7.11.24

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
CoraLite® Plus 488
规格: 

-/ -

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经过测试的应用

Positive WB detected inMCF-7 cells, Jurkat cells, HEK-293 cells, HepG2 cells, C6 cells, NIH/3T3 cells, rat brain tissue
Positive IHC detected inhuman cervical cancer tissue, human intrahepatic cholangiocarcinoma tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells
Positive FC (Intra) detected inHepG2 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:150-1:600
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

83533-1-RR targets ERK1/2 in WB, IHC, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, FC (Intra), ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 ERK1/2 fusion protein Ag20350 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 mitogen-activated protein kinase 3
别名 ERK1, ERK, ERK2, ERK-1, EC:2.7.11.24
计算分子量 43 kDa
观测分子量 38-44 kDa
GenBank蛋白编号BC013992
基因名称 ERK1
Gene ID (NCBI) 5595
RRIDAB_3671158
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purfication
UNIPROT IDP27361
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

ERK1 and ERK2 belongs to the protein kinase superfamily. It is involved in both the initiation and regulation of meiosis, mitosis, and postmitotic functions in differentiated cells by phosphorylating a number of transcription factors such as ELK-1. ERK1/2 catalized the reaction: ATP + a protein = ADP + a phosphoprotein. It is activated by tyrosine phosphorylation in response to INS and NGF. This antibody can recognize both ERK1 and ERK2 with the molecular mass of 38-44 kDa.

实验方案

Product Specific Protocols
WB protocol for ERK1/2 antibody 83533-1-RRDownload protocol
IHC protocol for ERK1/2 antibody 83533-1-RRDownload protocol
IF protocol for ERK1/2 antibody 83533-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols