ERK1/2 Recombinant antibody
ERK1/2 Recombinant Antibody for WB, IF/ICC, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
Human, Mouse, Rat
Applications
WB, IF/ICC, ELISA
Conjugate
Unconjugated
CloneNo.
4P8
验证数据展示
经过测试的应用
Positive WB detected in | MCF-7 cells, Jurkat cells, HEK-293 cells, HepG2 cells, C6 cells, NIH/3T3 cells, rat brain tissue |
Positive IF detected in | HeLa cells |
推荐稀释比
Application | Dilution |
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Western Blot (WB) | WB : 1:500-1:2000 |
Immunofluorescence (IF) | IF : 1:150-1:600 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
The immunogen of 83533-1-RR is ERK1/2 Fusion Protein expressed in E. coli.
Tested Applications | WB, IF/ICC, ELISA Application Description |
Tested Reactivity | Human, Mouse, Rat |
Immunogen | ERK1/2 fusion protein Ag20350 种属同源性预测 |
Host / Isotype | Rabbit / IgG |
Class | Recombinant |
Type | Antibody |
Full Name | mitogen-activated protein kinase 3 |
Synonyms | ERK 1, ERK1, ERK1/2, ERT2, HS44KDAP, HUMKER1A, Insulin stimulated MAP2 kinase, MAP kinase 1, MAP kinase 3, MAP kinase isoform p44, MAPK 1, MAPK 3, MAPK3, p44 ERK1, p44 MAPK, P44ERK1, P44MAPK, PRKM3 |
Calculated Molecular Weight | 43 kDa |
Observed Molecular Weight | 38-44 kDa |
GenBank Accession Number | BC013992 |
Gene Symbol | MAPK3 |
Gene ID (NCBI) | 5595 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Protein A purfication |
UNIPROT ID | P27361 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
实验方案
Product Specific Protocols | |
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WB protocol for ERK1/2 antibody 83533-1-RR | Download protocol |
IF protocol for ERK1/2 antibody 83533-1-RR | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |