ERK1/2 Recombinant antibody
ERK1/2 Uni-rAbTM Recombinant Antibody for WB, IHC, IF/ICC, FC (Intra), ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
WB, IHC, IF/ICC, FC (Intra), ELISA
Conjugate
Unconjugated
CloneNo.
4P8
验证数据展示
经过测试的应用
Positive WB detected in | MCF-7 cells, Jurkat cells, HEK-293 cells, HepG2 cells, C6 cells, NIH/3T3 cells, rat brain tissue |
Positive IHC detected in | human cervical cancer tissue, human intrahepatic cholangiocarcinoma tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF/ICC detected in | HeLa cells |
Positive FC (Intra) detected in | HepG2 cells |
推荐稀释比
Application | Dilution |
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Western Blot (WB) | WB : 1:500-1:2000 |
Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
Immunofluorescence (IF)/ICC | IF/ICC : 1:150-1:600 |
Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
The immunogen of 83533-1-RR is ERK1/2 Fusion Protein expressed in E. coli.
Tested Applications | WB, IHC, IF/ICC, FC (Intra), ELISA Application Description |
Tested Reactivity | human, mouse, rat |
Immunogen | ERK1/2 fusion protein Ag20350 种属同源性预测 |
Host / Isotype | Rabbit / IgG |
Class | Recombinant |
Type | Antibody |
Full Name | mitogen-activated protein kinase 3 |
Synonyms | ERK1, ERK, ERK2, ERK-1, EC:2.7.11.24 |
Calculated Molecular Weight | 43 kDa |
Observed Molecular Weight | 38-44 kDa |
GenBank Accession Number | BC013992 |
Gene Symbol | ERK1 |
Gene ID (NCBI) | 5595 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Protein A purfication |
UNIPROT ID | P27361 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
实验方案
Product Specific Protocols | |
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WB protocol for ERK1/2 antibody 83533-1-RR | Download protocol |
IHC protocol for ERK1/2 antibody 83533-1-RR | Download protocol |
IF protocol for ERK1/2 antibody 83533-1-RR | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |