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验证数据展示

  • WB analysis using 66423-1-Ig (same clone as 66423-1-PBS)

    WB analysis using 66423-1-Ig (same clone as 66423-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66423-1-Ig (ERp57/ERp60 antibody) at dilution of 1:9700 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 66423-1-PBS in a different storage buffer formulation.

  • IF Staining of HepG2 using 66423-1-Ig (same clone as 66423-1-PBS)

    IF Staining of HepG2 using 66423-1-Ig (same clone as 66423-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using ERp57/ERp60 antibody (66423-1-Ig, Clone: 3G4G7 ) at dilution of 1:1000 and Multi-rAb CoraLite® Plus 594-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (Cat.NO. RGAM004 ). This data was developed using the same antibody clone with 66423-1-PBS in a different storage buffer formulation.

  • IF Staining of HEK-293 using 66423-1-Ig (same clone as 66423-1-PBS)

    IF Staining of HEK-293 using 66423-1-Ig (same clone as 66423-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed HEK-293 cells using ERp57/ERp60 antibody (66423-1-Ig, Clone: 3G4G7 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66423-1-PBS in a different storage buffer formulation.

  • IHC staining of human lung cancer using 66423-1-Ig (same clone as 66423-1-PBS)

    IHC staining of human lung cancer using 66423-1-Ig (same clone as 66423-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66423-1-Ig (ERp57/ERp60 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66423-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with PDIA3 antibody included
  • IHC staining of human lung cancer using 66423-1-Ig (same clone as 66423-1-PBS)

    IHC staining of human lung cancer using 66423-1-Ig (same clone as 66423-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66423-1-Ig (ERp57/ERp60 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66423-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with PDIA3 antibody included

ERp57/ERp60 Monoclonal antibody, PBS Only

ERp57/ERp60 Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 66423-1-PBS

产品说明书

CloneNo. 3G4G7

宿主/亚型

Mouse / IgG1

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, Indirect ELISA

别名

ERp57, PDIA3, ER60, EC:5.3.4.1, Disulfide isomerase ER-60

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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Planning an IHC experiment? We recommend our IHCeasy PDIA3 Ready-To-Use IHC Kit. PDIA3 primary antibody included.

产品信息

66423-1-PBS targets ERp57/ERp60 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 ERp57/ERp60 fusion protein Ag8741 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 protein disulfide isomerase family A, member 3
别名 ERp57, PDIA3, ER60, EC:5.3.4.1, Disulfide isomerase ER-60
计算分子量 505 aa, 57 kDa
观测分子量 57 kDa, 60 kDa
GenBank蛋白编号BC014433
基因名称 PDIA3
Gene ID (NCBI) 2923
RRIDAB_2881795
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein G purification
UNIPROT IDP30101
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

PDIA3, also named as P58, ER60, ERp57, ERp60, ERp61, GRP57, GRP58 and PI-PLC, is a member of the PDI family, participates in the oxidation, reduction, and isomerization of disulfide bonds for correct folding of secretory proteins before modification and transport in the endoplasmic reticulum. It is associated with apoptosis or inhibition of cancer cell growth. PDIA3 was once thought to be a phospholipase; however, it has been demonstrated that this protein actually has protein disulfide isomerase activity. It is thought that complexes of lectins and PDIA3 mediate protein folding by promoting formation of disulfide bonds in their glycoprotein substrates.