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验证数据展示

  • WB analysis using 66423-1-Ig

    WB analysis using 66423-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 66423-1-Ig (ERp57/ERp60 antibody) at dilution of 1:9700 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.

  • IF Staining of HepG2 using 66423-1-Ig

    IF Staining of HepG2 using 66423-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using ERp57/ERp60 antibody (66423-1-Ig, Clone: 3G4G7 ) at dilution of 1:1000 and Multi-rAb CoraLite® Plus 594-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (Cat.NO. RGAM004 ).

  • IF Staining of HEK-293 using 66423-1-Ig

    IF Staining of HEK-293 using 66423-1-Ig

    Immunofluorescent analysis of (-20°C Methanol) fixed HEK-293 cells using ERp57/ERp60 antibody (66423-1-Ig, Clone: 3G4G7 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

  • IHC staining of human lung cancer using 66423-1-Ig

    IHC staining of human lung cancer using 66423-1-Ig

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66423-1-Ig (ERp57/ERp60 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with PDIA3 antibody included
  • IHC staining of human lung cancer using 66423-1-Ig

    IHC staining of human lung cancer using 66423-1-Ig

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66423-1-Ig (ERp57/ERp60 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with PDIA3 antibody included

ERp57/ERp60 Monoclonal antibody

ERp57/ERp60 Monoclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 66423-1-Ig

产品说明书

CloneNo. 3G4G7

宿主/亚型

Mouse / IgG1

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, ELISA

别名

ERp57, PDIA3, ER60, EC:5.3.4.1, Disulfide isomerase ER-60

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inU2OS cells, HeLa cells, HEK-293 cells, Jurkat cells, RAW 264.7 cells, LNCaP cells, K-562 cells, HSC-T6 cells, PC-12 cells, NIH/3T3 cells
Positive IHC detected inhuman lung cancer tissue, human liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHEK-293 cells, HepG2 cells
Planning an IHC experiment? We recommend our IHCeasy PDIA3 Ready-To-Use IHC Kit. PDIA3 primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 and CoraLite®594 conjugated versions of this antibody.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:2000-1:20000
Immunohistochemistry (IHC)IHC : 1:200-1:800
Immunofluorescence (IF)/ICCIF/ICC : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

66423-1-Ig targets ERp57/ERp60 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 ERp57/ERp60 fusion protein Ag8741 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 protein disulfide isomerase family A, member 3
别名 ERp57, PDIA3, ER60, EC:5.3.4.1, Disulfide isomerase ER-60
计算分子量 505 aa, 57 kDa
观测分子量 57 kDa, 60 kDa
GenBank蛋白编号BC014433
基因名称 PDIA3
Gene ID (NCBI) 2923
RRIDAB_2881795
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein G purification
UNIPROT IDP30101
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

PDIA3, also named as P58, ER60, ERp57, ERp60, ERp61, GRP57, GRP58 and PI-PLC, is a member of the PDI family, participates in the oxidation, reduction, and isomerization of disulfide bonds for correct folding of secretory proteins before modification and transport in the endoplasmic reticulum. It is associated with apoptosis or inhibition of cancer cell growth. PDIA3 was once thought to be a phospholipase; however, it has been demonstrated that this protein actually has protein disulfide isomerase activity. It is thought that complexes of lectins and PDIA3 mediate protein folding by promoting formation of disulfide bonds in their glycoprotein substrates.

实验方案

Product Specific Protocols
WB protocol for ERp57/ERp60 antibody 66423-1-IgDownload protocol
IHC protocol for ERp57/ERp60 antibody 66423-1-IgDownload protocol
IF protocol for ERp57/ERp60 antibody 66423-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols