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CoraLite®594-conjugated ERp57/ERp60 Monoclonal antibody

ERp57/ERp60 Monoclonal Antibody for IF/ICC

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

IF/ICC

Conjugate

CoraLite®594 Fluorescent Dye

CloneNo.

3G4G7

Cat no : CL594-66423

Print datasheet

Synonyms

58 kDa microsomal protein, Cell and organelle markers, Disulfide isomerase ER 60, ER protein 57, ER protein 60, ER60, ERp57, ERp60, ERp61, GRP57, GRP58, HsT17083, P58, PDIA3, PI PLC, Protein disulfide isomerase A3

验证数据展示

  • IF Staining of U2OS using CL594-66423

    IF Staining of U2OS using CL594-66423

    Immunofluorescent analysis of (4% PFA) fixed U2OS cells using CoraLite®594 ERp57/ERp60 antibody (CL594-66423, Clone: 3G4G7 ) at dilution of 1:200.

经过测试的应用

Positive IF/ICC detected inU2OS cells
Planning an IHC experiment? We recommend our IHCeasy PDIA3 Ready-To-Use IHC Kit. PDIA3 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 66423-1-Ig

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL594-66423 targets ERp57/ERp60 in IF/ICC applications and shows reactivity with human, mouse, rat samples.

Tested Applications IF/ICC Application Description
Tested Reactivity human, mouse, rat
Immunogen ERp57/ERp60 fusion protein Ag8741 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name protein disulfide isomerase family A, member 3
Synonyms 58 kDa microsomal protein, Cell and organelle markers, Disulfide isomerase ER 60, ER protein 57, ER protein 60, ER60, ERp57, ERp60, ERp61, GRP57, GRP58, HsT17083, P58, PDIA3, PI PLC, Protein disulfide isomerase A3
Calculated Molecular Weight 505 aa, 57 kDa
Observed Molecular Weight 57 kDa, 60 kDa
GenBank Accession NumberBC014433
Gene Symbol PDIA3
Gene ID (NCBI) 2923
Conjugate CoraLite®594 Fluorescent Dye
Excitation/Emission Maxima Wavelengths588 nm / 604 nm
Form Liquid
Purification MethodProtein G purification
UNIPROT IDP30101
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

PDIA3, also named as P58, ER60, ERp57, ERp60, ERp61, GRP57, GRP58 and PI-PLC, is a member of the PDI family, participates in the oxidation, reduction, and isomerization of disulfide bonds for correct folding of secretory proteins before modification and transport in the endoplasmic reticulum. It is associated with apoptosis or inhibition of cancer cell growth. PDIA3 was once thought to be a phospholipase; however, it has been demonstrated that this protein actually has protein disulfide isomerase activity. It is thought that complexes of lectins and PDIA3 mediate protein folding by promoting formation of disulfide bonds in their glycoprotein substrates.

实验方案

Product Specific Protocols
IF protocol for CL594 ERp57/ERp60 antibody CL594-66423Download protocol
Standard Protocols
Click here to view our Standard Protocols