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验证数据展示

  • IF Staining of HEK-293 using CL488-66423

    IF Staining of HEK-293 using CL488-66423

    Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using CL488-66423 (ERp57/ERp60 antibody) at dilution of 1:100.

CoraLite® Plus 488-conjugated ERp57/ERp60 Monoclonal antibody

ERp57/ERp60 Monoclonal Antibody for IF/ICC
Cat No. CL488-66423

产品说明书

CloneNo. 3G4G7

宿主/亚型

Mouse / IgG1

种属反应性

human, mouse, rat

应用

IF/ICC

别名

58 kDa microsomal protein, Cell and organelle markers, Disulfide isomerase ER 60, endoplasmic reticulum marker, ER marker, ER protein 57, ER protein 60, ER60, ERp57, ERp60, ERp61, GRP57, GRP58, HsT17083, P58, PDIA3, PI PLC, Protein disulfide isomerase A3

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite® Plus 488
规格: 

-/ -

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经过测试的应用

Positive IF/ICC detected inHEK-293 cells
Planning an IHC experiment? We recommend our IHCeasy PDIA3 Ready-To-Use IHC Kit. PDIA3 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 66423-1-Ig

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-66423 targets ERp57/ERp60 in IF/ICC applications and shows reactivity with human, mouse, rat samples.

经测试应用 IF/ICC Application Description
经测试反应性 human, mouse, rat
免疫原 ERp57/ERp60 fusion protein Ag8741 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 protein disulfide isomerase family A, member 3
别名 58 kDa microsomal protein, Cell and organelle markers, Disulfide isomerase ER 60, endoplasmic reticulum marker, ER marker, ER protein 57, ER protein 60, ER60, ERp57, ERp60, ERp61, GRP57, GRP58, HsT17083, P58, PDIA3, PI PLC, Protein disulfide isomerase A3
计算分子量 505 aa, 57 kDa
观测分子量 57 kDa, 60 kDa
GenBank蛋白编号BC014433
基因名称 PDIA3
Gene ID (NCBI) 2923
RRIDAB_2883334
偶联类型 CoraLite® Plus 488 Fluorescent Dye
最大激发/发射波长493 nm / 522 nm
形式 Liquid
纯化方式Protein G purification
UNIPROT IDP30101
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

PDIA3, also named as P58, ER60, ERp57, ERp60, ERp61, GRP57, GRP58 and PI-PLC, is a member of the PDI family, participates in the oxidation, reduction, and isomerization of disulfide bonds for correct folding of secretory proteins before modification and transport in the endoplasmic reticulum. It is associated with apoptosis or inhibition of cancer cell growth. PDIA3 was once thought to be a phospholipase; however, it has been demonstrated that this protein actually has protein disulfide isomerase activity. It is thought that complexes of lectins and PDIA3 mediate protein folding by promoting formation of disulfide bonds in their glycoprotein substrates.

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 ERp57/ERp60 antibody CL488-66423Download protocol
Standard Protocols
Click here to view our Standard Protocols