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验证数据展示

  • FC experiment of HepG2 using CL647-15967

    FC experiment of HepG2 using CL647-15967

    1X10^6 HepG2 cells were intracellularly stained with 0.2 ug CoraLite® Plus 647 Anti-Human ERp57/ERp60 (CL647-15967) (red), or 0.2 ug isotype control (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

  • Featured Product
  • KD/KO Validated

CoraLite® Plus 647-conjugated ERp57/ERp60 Polyclonal antibody

ERp57/ERp60 Polyclonal Antibody for FC (Intra)
Cat No. CL647-15967

Print datasheet

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

FC (Intra)

Synonyms

ERp57, PDIA3, ERp60, ER60, EC:5.3.4.1

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  CoraLite® Plus 647
Size: 

-/ -

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经过测试的应用

Positive FC (Intra) detected inHepG2 cells
Planning an IHC experiment? We recommend our IHCeasy PDIA3 Ready-To-Use IHC Kit. PDIA3 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 15967-1-AP

推荐稀释比

ApplicationDilution
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.20 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL647-15967 targets ERp57/ERp60 in FC (Intra) applications and shows reactivity with human, mouse, rat samples.

Tested Applications FC (Intra) Application Description
Tested Reactivity human, mouse, rat
Immunogen ERp57/ERp60 fusion protein Ag8741 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name protein disulfide isomerase family A, member 3
Synonyms ERp57, PDIA3, ERp60, ER60, EC:5.3.4.1
Calculated Molecular Weight 505 aa, 57 kDa
Observed Molecular Weight 57 kDa
GenBank Accession NumberBC014433
Gene Symbol PDIA3
Gene ID (NCBI) 2923
RRIDAB_2934920
Conjugate CoraLite® Plus 647 Fluorescent Dye
Excitation/Emission Maxima Wavelengths654 nm / 674 nm
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP30101
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

PDIA3, also named as P58, ER60, ERp57, ERp60, ERp61, GRP57, GRP58 and PI-PLC, is a member of the PDI family, participates in the oxidation, reduction, and isomerization of disulfide bonds for correct folding of secretory proteins before modification and transport in the endoplasmic reticulum. It is associated with apoptosis or inhibition of cancer cell growth. PDIA3 was once thought to be a phospholipase; however, it has been demonstrated that this protein actually has protein disulfide isomerase activity. It is thought that complexes of lectins and PDIA3 mediate protein folding by promoting formation of disulfide bonds in their glycoprotein substrates.

实验方案

Product Specific Protocols
FC protocol for CL Plus 647 ERp57/ERp60 antibody CL647-15967Download protocol
Standard Protocols
Click here to view our Standard Protocols