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ERp72 Monoclonal antibody, PBS Only

ERp72 Monoclonal Antibody for WB, IHC, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

WB, IHC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1D5F3

Cat no : 66365-1-PBS

Print datasheet

Synonyms

Cell and organelle markers, endoplasmic reticulum marker, ER marker, ER protein 70, ER protein 72, ERp 72, ERP70, ERp72, PDIA4, Protein disulfide isomerase A4

验证数据展示

  • WB analysis using 66365-1-Ig (same clone as 66365-1-PBS)

    WB analysis using 66365-1-Ig (same clone as 66365-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66365-1-Ig (ERp72 antibody) at dilution of 1:4900 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Beta Actin Monoclonal antibody (HRP-66009) as loading control. This data was developed using the same antibody clone with 66365-1-PBS in a different storage buffer formulation.

  • IHC staining of human liver cancer using 66365-1-Ig (same clone as 66365-1-PBS)

    IHC staining of human liver cancer using 66365-1-Ig (same clone as 66365-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66365-1-Ig (ERp72 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66365-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with PDIA4/ERP72 antibody included
Planning an IHC experiment? We recommend our IHCeasy PDIA4/ERP72 Ready-To-Use IHC Kit. PDIA4/ERP72 primary antibody included.

产品信息

66365-1-PBS targets ERp72 in WB, IHC, Indirect ELISA applications and shows reactivity with human samples.

Tested Applications WB, IHC, Indirect ELISA Application Description
Tested Reactivity human
Immunogen ERp72 fusion protein Ag6873 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name protein disulfide isomerase family A, member 4
Synonyms Cell and organelle markers, endoplasmic reticulum marker, ER marker, ER protein 70, ER protein 72, ERp 72, ERP70, ERp72, PDIA4, Protein disulfide isomerase A4
Calculated Molecular Weight 73 kDa
Observed Molecular Weight 72 kDa
GenBank Accession NumberBC000425
Gene Symbol ERp72
Gene ID (NCBI) 9601
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDP13667
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

PDIA4 (Protein disulfide-isomerase A4) is also named ERP70, ERP72, and belongs to the protein disulfide isomerase family. It catalyzes the rearrangement of -S-S- bonds in proteins. ERp72 is a soluble protein localized in the ER lumen and contains the COOH-terminal retention signal, KEEL. There are 6 cysteine residues in the amino acid sequences of mouse and human ERp72. All of the cysteine residues occur in the internal thioredoxin motif, CGHC(PMID:15475357). The full-length protein has a signal peptide with 20 amino acids.