验证数据展示
经过测试的应用
| Positive WB detected in | A431 cells, JAR cells, HEK-293 cells |
| Positive IHC detected in | human liver cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:10000 |
| Immunohistochemistry (IHC) | IHC : 1:250-1:1000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 1 publications below |
| IF | See 5 publications below |
产品信息
66365-1-Ig targets ERp72 in WB, IHC, IF, ELISA applications and shows reactivity with human samples.
| Tested Applications | WB, IHC, ELISA Application Description |
| Cited Applications | WB, IF |
| Tested Reactivity | human |
| Cited Reactivity | human, mouse, rat |
| Immunogen | ERp72 fusion protein Ag6873 种属同源性预测 |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Full Name | protein disulfide isomerase family A, member 4 |
| Synonyms | Cell and organelle markers, endoplasmic reticulum marker, ER marker, ER protein 70, ER protein 72, ERp 72, ERP70, ERp72, PDIA4, Protein disulfide isomerase A4 |
| Calculated Molecular Weight | 73 kDa |
| Observed Molecular Weight | 72 kDa |
| GenBank Accession Number | BC000425 |
| Gene Symbol | ERp72 |
| Gene ID (NCBI) | 9601 |
| RRID | AB_2881745 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein G purification |
| UNIPROT ID | P13667 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
PDIA4 (Protein disulfide-isomerase A4) is also named ERP70, ERP72, and belongs to the protein disulfide isomerase family. It catalyzes the rearrangement of -S-S- bonds in proteins. ERp72 is a soluble protein localized in the ER lumen and contains the COOH-terminal retention signal, KEEL. There are 6 cysteine residues in the amino acid sequences of mouse and human ERp72. All of the cysteine residues occur in the internal thioredoxin motif, CGHC(PMID:15475357). The full-length protein has a signal peptide with 20 amino acids.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for ERp72 antibody 66365-1-Ig | Download protocol |
| IHC protocol for ERp72 antibody 66365-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Cell Rep The ubiquitin E3 ligase TRIM10 promotes STING aggregation and activation in the Golgi apparatus | ||
Hypertension Upregulation of Piezo1 (Piezo Type Mechanosensitive Ion Channel Component 1) Enhances the Intracellular Free Calcium in Pulmonary Arterial Smooth Muscle Cells From Idiopathic Pulmonary Arterial Hypertension Patients. | ||
Front Mol Biosci Glycosylation at Asn254 Is Required for the Activation of the PDGF-C Protein. | ||
J Exp Clin Cancer Res 14-3-3ζ inhibits heme oxygenase-1 (HO-1) degradation and promotes hepatocellular carcinoma proliferation: involvement of STAT3 signaling. | ||
Biomed Pharmacother AKAP1 alleviates VSMC phenotypic modulation and neointima formation by inhibiting Drp1-dependent mitochondrial fission | ||
