ERp72 Monoclonal antibody

ERp72 Monoclonal Antibody for WB, IHC, ELISA
Cat No. 66365-1-Ig

产品说明书

CloneNo. 1D5F3

宿主/亚型

Mouse / IgG1

种属反应性

human and More (2)

应用

WB, IHC, ELISA and More (1)

Cell and organelle markers, endoplasmic reticulum marker, ER marker, ER protein 70, ER protein 72, ERp 72, ERP70, ERp72, PDIA4, Protein disulfide isomerase A4

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -


经过测试的应用

Positive WB detected inA431 cells, JAR cells, HEK-293 cells
Positive IHC detected inhuman liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy PDIA4/ERP72 Ready-To-Use IHC Kit. PDIA4/ERP72 primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 and CoraLite®594 conjugated versions of this antibody.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:2000-1:10000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

66365-1-Ig targets ERp72 in WB, IHC, IF, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, ELISA Application Description
文献引用应用WB, IF
经测试反应性 human
文献引用反应性human, mouse, rat
免疫原 ERp72 fusion protein Ag6873 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 protein disulfide isomerase family A, member 4
别名 Cell and organelle markers, endoplasmic reticulum marker, ER marker, ER protein 70, ER protein 72, ERp 72, ERP70, ERp72, PDIA4, Protein disulfide isomerase A4
计算分子量 73 kDa
观测分子量 72 kDa
GenBank蛋白编号BC000425
基因名称 ERp72
Gene ID (NCBI) 9601
RRIDAB_2881745
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein G purification
UNIPROT IDP13667
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

PDIA4 (Protein disulfide-isomerase A4) is also named ERP70, ERP72, and belongs to the protein disulfide isomerase family. It catalyzes the rearrangement of -S-S- bonds in proteins. ERp72 is a soluble protein localized in the ER lumen and contains the COOH-terminal retention signal, KEEL. There are 6 cysteine residues in the amino acid sequences of mouse and human ERp72. All of the cysteine residues occur in the internal thioredoxin motif, CGHC(PMID:15475357). The full-length protein has a signal peptide with 20 amino acids.

实验方案

Product Specific Protocols
WB protocol for ERp72 antibody 66365-1-IgDownload protocol
IHC protocol for ERp72 antibody 66365-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Cell Rep

The ubiquitin E3 ligase TRIM10 promotes STING aggregation and activation in the Golgi apparatus

Authors - Lingli Kong
humanIF

Hypertension

Upregulation of Piezo1 (Piezo Type Mechanosensitive Ion Channel Component 1) Enhances the Intracellular Free Calcium in Pulmonary Arterial Smooth Muscle Cells From Idiopathic Pulmonary Arterial Hypertension Patients.

Authors - Jing Liao
mouseIF

Front Mol Biosci

Glycosylation at Asn254 Is Required for the Activation of the PDGF-C Protein.

Authors - Wenjie Hu
humanIF

J Exp Clin Cancer Res

14-3-3ζ inhibits heme oxygenase-1 (HO-1) degradation and promotes hepatocellular carcinoma proliferation: involvement of STAT3 signaling.

Authors - Jia Song
human,ratIF

Biomed Pharmacother

AKAP1 alleviates VSMC phenotypic modulation and neointima formation by inhibiting Drp1-dependent mitochondrial fission

Authors - Jingwen Sun
humanIF

Brain

Heterozygous variants in USP25 cause genetic generalized epilepsy

Authors - Cui-Xia Fan
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