验证数据展示
经过测试的应用
| Positive IF/ICC detected in | HepG2 cells, HeLa cells |
Planning an IHC experiment? We recommend our IHCeasy PDIA4/ERP72 Ready-To-Use IHC Kit. PDIA4/ERP72 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 66365-1-Ig
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
CL594-66365 targets ERp72 in IF/ICC applications and shows reactivity with human samples.
| 经测试应用 | IF/ICC Application Description |
| 经测试反应性 | human |
| 免疫原 | ERp72 fusion protein Ag6873 种属同源性预测 |
| 宿主/亚型 | Mouse / IgG1 |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | protein disulfide isomerase family A, member 4 |
| 别名 | Cell and organelle markers, endoplasmic reticulum marker, ER marker, ER protein 70, ER protein 72, ERp 72, ERP70, ERP72, PDIA4, Protein disulfide isomerase A4 |
| 计算分子量 | 73 kDa |
| 观测分子量 | 72 kDa |
| GenBank蛋白编号 | BC000425 |
| 基因名称 | ERp72 |
| Gene ID (NCBI) | 9601 |
| RRID | AB_2883569 |
| 偶联类型 | CoraLite®594 Fluorescent Dye |
| 最大激发/发射波长 | 588 nm / 604 nm |
| 形式 | Liquid |
| 纯化方式 | Protein G purification |
| UNIPROT ID | P13667 |
| 储存缓冲液 | PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3 |
| 储存条件 | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
PDIA4 (Protein disulfide-isomerase A4) is also named ERP70, ERP72, and belongs to the protein disulfide isomerase family. It catalyzes the rearrangement of -S-S- bonds in proteins. ERp72 is a soluble protein localized in the ER lumen and contains the COOH-terminal retention signal, KEEL. There are 6 cysteine residues in the amino acid sequences of mouse and human ERp72. All of the cysteine residues occur in the internal thioredoxin motif, CGHC(PMID:15475357). The full-length protein has a signal peptide with 20 amino acids.
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for CL594 ERp72 antibody CL594-66365 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
