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  • Featured Product
  • KD/KO Validated

ESR2 Monoclonal antibody, PBS Only

ESR2 Monoclonal Antibody for WB, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

WB, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

5D2C9

Cat no : 60197-1-PBS

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Synonyms

ER BETA, Erb, ERβ, ESR BETA, ESR2, ESRB, ESTRB, estrogen receptor 2 (ER beta), Estrogen receptor beta, NR3A2

验证数据展示

  • WB analysis of MCF-7 using 60197-1-Ig (same clone as 60197-1-PBS)

    WB analysis of MCF-7 using 60197-1-Ig (same clone as 60197-1-PBS)

    MCF-7 cells were subjected to SDS PAGE followed by western blot with 60197-1-Ig (ESR2 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60197-1-PBS in a different storage buffer formulation.

产品信息

60197-1-PBS targets ESR2 in WB, Indirect ELISA applications and shows reactivity with human samples.

Tested Applications WB, Indirect ELISA Application Description
Tested Reactivity human
Immunogen ESR2 fusion protein Ag5103 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name estrogen receptor 2 (ER beta)
Synonyms ER BETA, Erb, ERβ, ESR BETA, ESR2, ESRB, ESTRB, estrogen receptor 2 (ER beta), Estrogen receptor beta, NR3A2
Calculated Molecular Weight 59 kDa
Observed Molecular Weight 50-60 kDa
GenBank Accession NumberBC024181
Gene Symbol ESR2
Gene ID (NCBI) 2100
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDQ92731
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Estrogen receptor-beta (ESR2) is a member of the superfamily of nuclear receptors, which can transduce extracellular signals into transcriptional responses. It binds estrogens with an affinity similar to that of ESR1, and activates expression of reporter genes containing estrogen response elements (ERE) in an estrogen-dependent manner. DNA-binding by ESR1 and ESR2 is rapidly lost at 37 degrees Celsius in the absence of ligand while in the presence of 17 beta-estradiol and 4-hydroxy-tamoxifen loss in DNA-binding at elevated temperature is more gradual. ESR2 exists various isoform and range of calculated molecular weight of isoforms is 50-60 kDa. The experiment detected two bands for ESR2 in isolated human germ cells, one at 60 kDa and a weaker one at 50 kDa (PMID: 14766008).