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ESR2 Recombinant antibody, PBS Only (Capture/Detector)

ESR2 Uni-rAbTM Recombinant Antibody for IHC, IF/ICC, FC (Intra), Cytometric bead array, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

IHC, IF/ICC, FC (Intra), Cytometric bead array, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

240412F2

Cat no : 83447-3-PBS

Print datasheet

Synonyms

ESTRB, ERβ, ER-beta, Erb, ER BETA

验证数据展示

  • FC experiment of MCF-7 using 83447-3-RR (same clone as 83447-3-PBS)

    FC experiment of MCF-7 using 83447-3-RR (same clone as 83447-3-PBS)

    1x10^6 MCF-7 cells were intracellularly stained with 0.25 ug Esr2 Recombinant Antibody (83447-3-RR, Clone:240412F2) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 83447-3-PBS in a different storage buffer formulation.

  • IF Staining of HepG2 using 83447-3-RR (same clone as 83447-3-PBS)

    IF Staining of HepG2 using 83447-3-RR (same clone as 83447-3-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using ESR2 antibody (83447-3-RR, Clone: 240412F2 ) at dilution of 1:800 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-Phalloidin (red). This data was developed using the same antibody clone with 83447-3-PBS in a different storage buffer formulation.

  • IHC staining of mouse testis using 83447-3-RR (same clone as 83447-3-PBS)

    IHC staining of mouse testis using 83447-3-RR (same clone as 83447-3-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 83447-3-RR (ESR2 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83447-3-PBS in a different storage buffer formulation.

  • IHC staining of mouse ovary using 83447-3-RR (same clone as 83447-3-PBS)

    IHC staining of mouse ovary using 83447-3-RR (same clone as 83447-3-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse ovary tissue slide using 83447-3-RR (ESR2 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83447-3-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP00441-1

    Cytometric bead array standard curve of MP00441-1

    Cytometric bead array standard curve of MP00441-1, ESR2 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83447-2-PBS. Detection antibody: 83447-3-PBS. Standard: Ag5103. Range: 0.781-100 ng/mL.

  • Cytometric bead array standard curve of MP00441-2

    Cytometric bead array standard curve of MP00441-2

    Cytometric bead array standard curve of MP00441-2, ESR2 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83447-3-PBS. Detection antibody: 83447-1-PBS. Standard: Ag5103. Range: 0.781-50 ng/mL.

  • Affinity and Kinetic Characterization of 83447-3-PBS

    Affinity and Kinetic Characterization of 83447-3-PBS

    Biolayer interferometry (BLl) kinetic assay of 83447-3-PBS against Human ESR2 was performed. The affinity constant is 16.6 pM.

  • Affinity and Kinetic Characterization of 83447-3-RR

    Affinity and Kinetic Characterization of 83447-3-RR

    Biolayer interferometry (BLl) kinetic assays of 83447-3-RR against Human ESR2 were performed. The affinity constant is 0.22 nM.

产品信息

83447-3-PBS targets ESR2 as part of a matched antibody pair:

MP00441-1: 83447-2-PBS capture and 83447-3-PBS detection (validated in Cytometric bead array)

MP00441-2: 83447-3-PBS capture and 83447-1-PBS detection (validated in Cytometric bead array)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Applications IHC, IF/ICC, FC (Intra), Cytometric bead array, Indirect ELISA Application Description
Tested Reactivity human, mouse
Immunogen ESR2 fusion protein Ag5103 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name estrogen receptor 2 (ER beta)
Synonyms ESTRB, ERβ, ER-beta, Erb, ER BETA
Calculated Molecular Weight 59 kDa
GenBank Accession NumberBC024181
Gene Symbol ESR2
Gene ID (NCBI) 2100
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ92731
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Estrogen receptor-beta (ESR2) is a member of the superfamily of nuclear receptors, which can transduce extracellular signals into transcriptional responses. It binds estrogens with an affinity similar to that of ESR1, and activates expression of reporter genes containing estrogen response elements (ERE) in an estrogen-dependent manner. DNA-binding by ESR1 and ESR2 is rapidly lost at 37 degrees Celsius in the absence of ligand while in the presence of 17 beta-estradiol and 4-hydroxy-tamoxifen loss in DNA-binding at elevated temperature is more gradual.