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ETS1 Polyclonal antibody

ETS1 Polyclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

IF, IHC, WB, ELISA

Conjugate

Unconjugated

Cat No : 12118-1-AP

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Synonyms

ETS 1, ETS1, EWSR2, p54, Protein C ets 1



经过测试的应用

Positive WB detected inJurkat cells, NIH/3T3 cells, mouse thymus tissue
Positive IHC detected inrat spleen tissue, mouse spleen tissue, rat lung tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inA431 cells
Planning an IHC experiment? We recommend our IHCeasy ETS1 Ready-To-Use IHC Kit. ETS1 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
Immunofluorescence (IF)IF : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

12118-1-AP targets ETS1 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications IF, IHC, WB, ELISA
Cited ApplicationsWB
Tested Reactivity human, mouse, rat
Cited Reactivityhuman, rat, sheep
Immunogen ETS1 fusion protein Ag2127 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name v-ets erythroblastosis virus E26 oncogene homolog 1 (avian)
Synonyms ETS 1, ETS1, EWSR2, p54, Protein C ets 1
Calculated Molecular Weight 441 aa, 50 kDa
Observed Molecular Weight 50 kDa
GenBank Accession NumberBC017314
Gene Symbol ETS1
Gene ID (NCBI) 2113
RRIDAB_10664925
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP14921
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

ETS1 is a member of the ETS family of transcription factors, which are defined by the presence of a conserved ETS DNA-binding domain that recognizes the core consensus DNA sequence GGAA/T in target genes. ETS1 is a nuclear protein and primarily acts as a transcriptional activator, though it can also repress gene transcription. Since it is primarily expressed in lymphocytes, it plays several critical roles in immunity. In addition, it is important for angiogenesis. Since cells express several Ets proteins at the same time, functional redundancy is possible. This is particularly shown for the socalled housekeeping genes where several Ets factors were found to occupy the proximal promoters in vivo. The ETS1 protein exists as three isoforms: ETS1-p51, ETS1-p42, ETS1-p27 ranging in size from 27 to 51 kDa.

实验方案

Product Specific Protocols
WB protocol for ETS1 antibody 12118-1-APDownload protocol
IHC protocol for ETS1 antibody 12118-1-APDownload protocol
IF protocol for ETS1 antibody 12118-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Nat Commun

HIV reprograms host m 6 Am RNA methylome by viral Vpr protein-mediated degradation of PCIF1

Authors - Qiong Zhang
humanWB

Front Cell Dev Biol

rSjP40 Inhibited the Activity of Collagen Type I Promoter via Ets-1 in HSCs.

Authors - Jing Li
  • KD Validated
humanWB

Oncotarget

DOT1L promotes angiogenesis through cooperative regulation of VEGFR2 with ETS-1.

Authors - Yang Duan
humanWB

Lung

ETS1 Ameliorates Hyperoxia-Induced Bronchopulmonary Dysplasia in Mice by Activating Nrf2/HO-1 Mediated Ferroptosis

Authors - Min Yang
humanWB

Int J Biol Sci

The RNF26/CBX7 axis modulates the TNF pathway to promote cell proliferation and regulate sensitivity to TKIs in ccRCC.

Authors - Wentao Liu
ratWB

Commun Biol

The histone demthylase KDM3A protects the myocardium from ischemia/reperfusion injury via promotion of ETS1 expression.

Authors - Xin Guo
  • KD Validated