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  • Featured Product
  • KD/KO Validated

ETS1 Polyclonal antibody, PBS Only

ETS1 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

Cat no : 12118-1-PBS

Print datasheet

Synonyms

验证数据展示

  • WB analysis using 12118-1-AP (same clone as 12118-1-PBS)

    WB analysis using 12118-1-AP (same clone as 12118-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 12118-1-AP (ETS1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12118-1-PBS in a different storage buffer formulation.

  • WB analysis of Jurkat using 12118-1-AP (same clone as 12118-1-PBS)

    WB analysis of Jurkat using 12118-1-AP (same clone as 12118-1-PBS)

    WB result of ETS1 antibody (12118-1-AP; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-ETS1 transfected Jurkat cells. This data was developed using the same antibody clone with 12118-1-PBS in a different storage buffer formulation.

  • IHC staining of rat spleen using 12118-1-AP (same clone as 12118-1-PBS)

    IHC staining of rat spleen using 12118-1-AP (same clone as 12118-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat spleen tissue slide using 12118-1-AP (ETS1 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12118-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with ETS1 antibody included
  • IF Staining of A431 using 12118-1-AP (same clone as 12118-1-PBS)

    IF Staining of A431 using 12118-1-AP (same clone as 12118-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed A431 cells using ETS1 antibody (12118-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 12118-1-PBS in a different storage buffer formulation.

  • IHC staining of rat spleen using 12118-1-AP (same clone as 12118-1-PBS)

    IHC staining of rat spleen using 12118-1-AP (same clone as 12118-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat spleen tissue slide using 12118-1-AP (ETS1 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12118-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with ETS1 antibody included
Planning an IHC experiment? We recommend our IHCeasy ETS1 Ready-To-Use IHC Kit. ETS1 primary antibody included.

产品信息

12118-1-PBS targets ETS1 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, IF/ICC, Indirect ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen ETS1 fusion protein Ag2127 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name v-ets erythroblastosis virus E26 oncogene homolog 1 (avian)
Synonyms
Calculated Molecular Weight 441 aa, 50 kDa
Observed Molecular Weight 50 kDa
GenBank Accession NumberBC017314
Gene Symbol ETS1
Gene ID (NCBI) 2113
RRIDAB_10664925
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP14921
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

ETS1 is a member of the ETS family of transcription factors, which are defined by the presence of a conserved ETS DNA-binding domain that recognizes the core consensus DNA sequence GGAA/T in target genes. ETS1 is a nuclear protein and primarily acts as a transcriptional activator, though it can also repress gene transcription. Since it is primarily expressed in lymphocytes, it plays several critical roles in immunity. In addition, it is important for angiogenesis. Since cells express several Ets proteins at the same time, functional redundancy is possible. This is particularly shown for the socalled housekeeping genes where several Ets factors were found to occupy the proximal promoters in vivo. The ETS1 protein exists as three isoforms: ETS1-p51, ETS1-p42, ETS1-p27 ranging in size from 27 to 51 kDa.