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ETS1 Monoclonal antibody
ETS1 Monoclonal Antibody for WB, IHC, IF/ICC, ELISA
Host / Isotype
Mouse / IgG2b
Reactivity
human, rat
Applications
WB, IHC, IF/ICC, ELISA and More (1)
Conjugate
Unconjugated
CloneNo.
4F2D4
验证数据展示
经过测试的应用
| Positive WB detected in | HepG2 cells, Jurkat cells, Daudi cells, HSCT6 cells |
| Positive IHC detected in | rat spleen tissue, mouse lung tissue, mouse small intestine tissue, rat lung tissue, rat small intestine tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | A431 cells |
Planning an IHC experiment? We recommend our IHCeasy ETS1 Ready-To-Use IHC Kit. ETS1 primary antibody included.
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:5000 |
| Immunohistochemistry (IHC) | IHC : 1:1000-1:4000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:400-1:1600 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
66598-1-Ig targets ETS1 in WB, IHC, IF/ICC, ChIP, ELISA applications and shows reactivity with human, rat samples.
| Tested Applications | WB, IHC, IF/ICC, ELISA Application Description |
| Cited Applications | WB, IHC, ChIP |
| Tested Reactivity | human, rat |
| Cited Reactivity | human |
| Immunogen | ETS1 fusion protein Ag2127 种属同源性预测 |
| Host / Isotype | Mouse / IgG2b |
| Class | Monoclonal |
| Type | Antibody |
| Full Name | v-ets erythroblastosis virus E26 oncogene homolog 1 (avian) |
| Synonyms | Protein C-ets-1, EWSR2, ETS 1, 4F2D4 |
| Calculated Molecular Weight | 441 aa, 50 kDa |
| Observed Molecular Weight | 50 kDa |
| GenBank Accession Number | BC017314 |
| Gene Symbol | ETS1 |
| Gene ID (NCBI) | 2113 |
| RRID | AB_2881958 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | P14921 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
ETS1 is a member of the ETS family of transcription factors, which are defined by the presence of a conserved ETS DNA-binding domain that recognizes the core consensus DNA sequence GGAA/T in target genes. ETS1 is a nuclear protein and primarily acts as a transcriptional activator, though it can also repress gene transcription. Since it is primarily expressed in lymphocytes, it plays several critical roles in immunity. In addition, it is important for angiogenesis. Since cells express several Ets proteins at the same time, functional redundancy is possible. This is particularly shown for the socalled housekeeping genes where several Ets factors were found to occupy the proximal promoters in vivo.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for ETS1 antibody 66598-1-Ig | Download protocol |
| IHC protocol for ETS1 antibody 66598-1-Ig | Download protocol |
| IF protocol for ETS1 antibody 66598-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Mol Med ets1 associates with KMT5A to participate in high glucose-mediated EndMT via upregulation of PFN2 expression in diabetic nephropathy.
| ||
Blood Adv ETS1 is a novel transcriptional regulator of adult T-cell leukemia/lymphoma of North American descent | ||
Nat Microbiol FEAR antiviral response pathway is independent of interferons and countered by poxvirus proteins | ||
Int J Biol Sci ETS1-mediated Regulation of SOAT1 Enhances the Malignant Phenotype of Oral Squamous Cell Carcinoma and Induces Tumor-associated Macrophages M2-like Polarization |
