验证数据展示
经过测试的应用
| Positive WB detected in | HepG2 cells, Jurkat cells, Daudi cells, HSCT6 cells |
| Positive IHC detected in | rat spleen tissue, mouse lung tissue, mouse small intestine tissue, rat lung tissue, rat small intestine tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | A431 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:5000 |
| Immunohistochemistry (IHC) | IHC : 1:1000-1:4000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:400-1:1600 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
66598-1-Ig targets ETS1 in WB, IHC, IF/ICC, ChIP, ELISA applications and shows reactivity with human, rat samples.
| 经测试应用 | WB, IHC, IF/ICC, ELISA Application Description |
| 文献引用应用 | WB, IHC, ChIP |
| 经测试反应性 | human, rat |
| 文献引用反应性 | human, mouse |
| 免疫原 | ETS1 fusion protein Ag2127 种属同源性预测 |
| 宿主/亚型 | Mouse / IgG2b |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | v-ets erythroblastosis virus E26 oncogene homolog 1 (avian) |
| 别名 | Protein C-ets-1, EWSR2, ETS 1, 4F2D4 |
| 计算分子量 | 441 aa, 50 kDa |
| 观测分子量 | 50 kDa |
| GenBank蛋白编号 | BC017314 |
| 基因名称 | ETS1 |
| Gene ID (NCBI) | 2113 |
| RRID | AB_2881958 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | P14921 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
ETS1 is a member of the ETS family of transcription factors, which are defined by the presence of a conserved ETS DNA-binding domain that recognizes the core consensus DNA sequence GGAA/T in target genes. ETS1 is a nuclear protein and primarily acts as a transcriptional activator, though it can also repress gene transcription. Since it is primarily expressed in lymphocytes, it plays several critical roles in immunity. In addition, it is important for angiogenesis. Since cells express several Ets proteins at the same time, functional redundancy is possible. This is particularly shown for the socalled housekeeping genes where several Ets factors were found to occupy the proximal promoters in vivo.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for ETS1 antibody 66598-1-Ig | Download protocol |
| IHC protocol for ETS1 antibody 66598-1-Ig | Download protocol |
| IF protocol for ETS1 antibody 66598-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Mol Med ets1 associates with KMT5A to participate in high glucose-mediated EndMT via upregulation of PFN2 expression in diabetic nephropathy.
| ||
Blood Adv ETS1 is a novel transcriptional regulator of adult T-cell leukemia/lymphoma of North American descent | ||
Nat Microbiol FEAR antiviral response pathway is independent of interferons and countered by poxvirus proteins | ||
Int J Biol Sci ETS1-mediated Regulation of SOAT1 Enhances the Malignant Phenotype of Oral Squamous Cell Carcinoma and Induces Tumor-associated Macrophages M2-like Polarization | ||
Elife Neurons enhance blood-brain barrier function via upregulating claudin-5 and VE-cadherin expression due to glial cell line-derived neurotrophic factor secretion
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