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  • Featured Product
  • KD/KO Validated

ETS1 Monoclonal antibody, PBS Only

ETS1 Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

human, rat

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

4F2D4

Cat no : 66598-1-PBS

Print datasheet

Synonyms

Protein C-ets-1, EWSR2, ETS 1, 4F2D4

验证数据展示

  • WB analysis of HepG2 using 66598-1-Ig (same clone as 66598-1-PBS)

    WB analysis of HepG2 using 66598-1-Ig (same clone as 66598-1-PBS)

    HepG2 cells were subjected to SDS PAGE followed by western blot with 66598-1-Ig (ETS1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66598-1-PBS in a different storage buffer formulation.

  • IHC staining of rat spleen using 66598-1-Ig (same clone as 66598-1-PBS)

    IHC staining of rat spleen using 66598-1-Ig (same clone as 66598-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat spleen tissue slide using 66598-1-Ig (ETS1 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66598-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with ETS1 antibody included
  • IF Staining of A431 using 66598-1-Ig (same clone as 66598-1-PBS)

    IF Staining of A431 using 66598-1-Ig (same clone as 66598-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed A431 cells using ETS1 antibody (66598-1-Ig, Clone: 4F2D4 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 66598-1-PBS in a different storage buffer formulation.

  • IHC staining of rat spleen using 66598-1-Ig (same clone as 66598-1-PBS)

    IHC staining of rat spleen using 66598-1-Ig (same clone as 66598-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat spleen tissue slide using 66598-1-Ig (ETS1 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66598-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with ETS1 antibody included
Planning an IHC experiment? We recommend our IHCeasy ETS1 Ready-To-Use IHC Kit. ETS1 primary antibody included.

产品信息

66598-1-PBS targets ETS1 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, rat samples.

Tested Applications WB, IHC, IF/ICC, Indirect ELISA Application Description
Tested Reactivity human, rat
Immunogen ETS1 fusion protein Ag2127 种属同源性预测
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Full Name v-ets erythroblastosis virus E26 oncogene homolog 1 (avian)
Synonyms Protein C-ets-1, EWSR2, ETS 1, 4F2D4
Calculated Molecular Weight 441 aa, 50 kDa
Observed Molecular Weight 50 kDa
GenBank Accession NumberBC017314
Gene Symbol ETS1
Gene ID (NCBI) 2113
RRIDAB_2881958
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP14921
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

ETS1 is a member of the ETS family of transcription factors, which are defined by the presence of a conserved ETS DNA-binding domain that recognizes the core consensus DNA sequence GGAA/T in target genes. ETS1 is a nuclear protein and primarily acts as a transcriptional activator, though it can also repress gene transcription. Since it is primarily expressed in lymphocytes, it plays several critical roles in immunity. In addition, it is important for angiogenesis. Since cells express several Ets proteins at the same time, functional redundancy is possible. This is particularly shown for the socalled housekeeping genes where several Ets factors were found to occupy the proximal promoters in vivo.