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验证数据展示

  • WB analysis of HepG2 using 66598-1-Ig (same clone as 66598-1-PBS)

    WB analysis of HepG2 using 66598-1-Ig (same clone as 66598-1-PBS)

    HepG2 cells were subjected to SDS PAGE followed by western blot with 66598-1-Ig (ETS1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66598-1-PBS in a different storage buffer formulation.

  • IHC staining of rat spleen using 66598-1-Ig (same clone as 66598-1-PBS)

    IHC staining of rat spleen using 66598-1-Ig (same clone as 66598-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat spleen tissue slide using 66598-1-Ig (ETS1 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66598-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with ETS1 antibody included
  • IF Staining of A431 using 66598-1-Ig (same clone as 66598-1-PBS)

    IF Staining of A431 using 66598-1-Ig (same clone as 66598-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed A431 cells using ETS1 antibody (66598-1-Ig, Clone: 4F2D4 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 66598-1-PBS in a different storage buffer formulation.

  • IHC staining of rat spleen using 66598-1-Ig (same clone as 66598-1-PBS)

    IHC staining of rat spleen using 66598-1-Ig (same clone as 66598-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat spleen tissue slide using 66598-1-Ig (ETS1 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66598-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with ETS1 antibody included
  • Featured Product
  • KD/KO验证

ETS1 Monoclonal antibody, PBS Only

ETS1 Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 66598-1-PBS

产品说明书

CloneNo. 4F2D4

宿主/亚型

Mouse / IgG2b

种属反应性

human, rat

应用

WB, IHC, IF/ICC, Indirect ELISA

别名

Protein C-ets-1, EWSR2, ETS 1, 4F2D4

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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Planning an IHC experiment? We recommend our IHCeasy ETS1 Ready-To-Use IHC Kit. ETS1 primary antibody included.

产品信息

66598-1-PBS targets ETS1 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, rat samples.

经测试应用 WB, IHC, IF/ICC, Indirect ELISA Application Description
文献引用应用WB
经测试反应性 human, rat
文献引用反应性human
免疫原 ETS1 fusion protein Ag2127 种属同源性预测
宿主/亚型 Mouse / IgG2b
抗体类别 Monoclonal
产品类型 Antibody
全称 v-ets erythroblastosis virus E26 oncogene homolog 1 (avian)
别名 Protein C-ets-1, EWSR2, ETS 1, 4F2D4
计算分子量 441 aa, 50 kDa
观测分子量 50 kDa
GenBank蛋白编号BC017314
基因名称 ETS1
Gene ID (NCBI) 2113
RRIDAB_2881958
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP14921
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

ETS1 is a member of the ETS family of transcription factors, which are defined by the presence of a conserved ETS DNA-binding domain that recognizes the core consensus DNA sequence GGAA/T in target genes. ETS1 is a nuclear protein and primarily acts as a transcriptional activator, though it can also repress gene transcription. Since it is primarily expressed in lymphocytes, it plays several critical roles in immunity. In addition, it is important for angiogenesis. Since cells express several Ets proteins at the same time, functional redundancy is possible. This is particularly shown for the socalled housekeeping genes where several Ets factors were found to occupy the proximal promoters in vivo.