验证数据展示
经过测试的应用
| Positive WB detected in | K-562 cells, HeLa cells, MCF-7 cells, mouse kidney tissue, PC-3 cells |
| Positive IP detected in | HeLa cells |
| Positive IHC detected in | mouse spleen tissue, rat liver tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Planning an IHC experiment? We recommend our IHCeasy ETS2 Ready-To-Use IHC Kit. ETS2 primary antibody included.
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 3 publications below |
| IHC | See 1 publications below |
产品信息
12280-1-AP targets ETS2 in WB, IP, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IP, IHC, ELISA Application Description |
| 文献引用应用 | WB, IHC |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse |
| 免疫原 | ETS2 fusion protein Ag2929 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | v-ets erythroblastosis virus E26 oncogene homolog 2 (avian) |
| 别名 | ETS2, ETS2IT1, Protein C ets 2 |
| 计算分子量 | 469 aa, 53 kDa |
| 观测分子量 | 53 kDa |
| GenBank蛋白编号 | BC017040 |
| 基因名称 | ETS2 |
| Gene ID (NCBI) | 2114 |
| RRID | AB_10804755 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | P15036 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for ETS2 antibody 12280-1-AP | Download protocol |
| IHC protocol for ETS2 antibody 12280-1-AP | Download protocol |
| IP protocol for ETS2 antibody 12280-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Cell Death Dis The overexpression of Tipe2 in CRC cells suppresses survival while endogenous Tipe2 accelerates AOM/DSS induced-tumor initiation. | ||
Cell Death Dis A distal super-enhancer activates oncogenic ETS2 via recruiting MECOM in inflammatory bowel disease and colorectal cancer | ||
Gene Cellular senescence-Related genes define the immune microenvironment and molecular characteristics in severe asthma patients | ||
BMC Med Single-cell and spatial transcriptomics reveal metastasis mechanism and microenvironment remodeling of lymph node in osteosarcoma |
