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验证数据展示

  • IHC staining of human breast cancer using 67579-1-Ig

    IHC staining of human breast cancer using 67579-1-Ig

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67579-1-Ig (ETV6 antibody) at dilution of 1:100 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • WB analysis of Jurkat using 67579-1-Ig

    WB analysis of Jurkat using 67579-1-Ig

    Jurkat cells were subjected to SDS PAGE followed by western blot with 67579-1-Ig (ETV6 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • WB analysis of K-562 using 67579-1-Ig

    WB analysis of K-562 using 67579-1-Ig

    K-562 cells were subjected to SDS PAGE followed by western blot with 67579-1-Ig (ETV6 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

ETV6 Monoclonal antibody

ETV6 Monoclonal Antibody for WB, IHC, ELISA
Cat No. 67579-1-Ig

产品说明书

CloneNo. 2A5A2

宿主/亚型

Mouse / IgG1

种属反应性

Human

应用

WB, IHC, ELISA

别名

ETS related protein Tel1, ETS translocation variant 6, ets variant 6, ETV6, TEL, TEL/ABL, TEL1, Transcription factor ETV6

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inJurkat cells, U2OS cells, A431 cells, K-562 cells, THP-1 cells
Positive IHC detected inhuman breast cancer tissue, human lung cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

67579-1-Ig targets ETV6 in WB, IHC, ELISA applications and shows reactivity with Human samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 Human
免疫原 ETV6 fusion protein Ag30054 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 ets variant 6
别名 ETS related protein Tel1, ETS translocation variant 6, ets variant 6, ETV6, TEL, TEL/ABL, TEL1, Transcription factor ETV6
计算分子量 452 aa, 53 kDa
观测分子量60 kDa
GenBank蛋白编号BC043399
基因名称 ETV6
Gene ID (NCBI) 2120
RRIDAB_2918493
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein G purification
UNIPROT IDP41212
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Aliquoting is unnecessary for -20oC storage.

背景介绍

ETV6(ETS Variant Transcription Factor 6), also known as TEL, is a Protein Coding gene. ETV6 is a member of the ETS family of transcription factors encoded by the ETS variant 6 gene (ETV6), located on chromosome 12p13.2. ETV6 protein contains 3 domains: a) the pointed N-terminal (i.e. PNT) domain which forms oligomer partners with itself as well as other transcription factors (e.g. FLI1) and is required for ETV6's transcriptional repressing activity; b) the central regulatory domain; and c) the C-terminal DNA-binding domain(PMID: 22578774). ETV6 is highly expressed in early hematopoietic progenitor cells(HPCs) and is essential for hematopoiesis in the bone marrow(PMID: 31248877).

实验方案

Product Specific Protocols
WB protocol for ETV6 antibody 67579-1-IgDownload protocol
IHC protocol for ETV6 antibody 67579-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols