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ETV6 Monoclonal antibody

ETV6 Monoclonal Antibody for WB, IHC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

2A5A2

Cat no : 67579-1-Ig

Print datasheet

Synonyms

ETS related protein Tel1, ETS translocation variant 6, ets variant 6, ETV6, TEL, TEL/ABL, TEL1, Transcription factor ETV6

验证数据展示

  • IHC staining of human breast cancer using 67579-1-Ig

    IHC staining of human breast cancer using 67579-1-Ig

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67579-1-Ig (ETV6 antibody) at dilution of 1:100 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • WB analysis of Jurkat using 67579-1-Ig

    WB analysis of Jurkat using 67579-1-Ig

    Jurkat cells were subjected to SDS PAGE followed by western blot with 67579-1-Ig (ETV6 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • WB analysis of K-562 using 67579-1-Ig

    WB analysis of K-562 using 67579-1-Ig

    K-562 cells were subjected to SDS PAGE followed by western blot with 67579-1-Ig (ETV6 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

经过测试的应用

Positive WB detected inJurkat cells, U2OS cells, A431 cells, K-562 cells, THP-1 cells
Positive IHC detected inhuman breast cancer tissue, human lung cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

67579-1-Ig targets ETV6 in WB, IHC, ELISA applications and shows reactivity with Human samples.

Tested Applications WB, IHC, ELISA Application Description
Tested Reactivity Human
Immunogen ETV6 fusion protein Ag30054 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name ets variant 6
Synonyms ETS related protein Tel1, ETS translocation variant 6, ets variant 6, ETV6, TEL, TEL/ABL, TEL1, Transcription factor ETV6
Calculated Molecular Weight 452 aa, 53 kDa
Observed Molecular Weight60 kDa
GenBank Accession NumberBC043399
Gene Symbol ETV6
Gene ID (NCBI) 2120
RRIDAB_2918493
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDP41212
Storage Buffer PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Aliquoting is unnecessary for -20oC storage.

背景介绍

ETV6(ETS Variant Transcription Factor 6), also known as TEL, is a Protein Coding gene. ETV6 is a member of the ETS family of transcription factors encoded by the ETS variant 6 gene (ETV6), located on chromosome 12p13.2. ETV6 protein contains 3 domains: a) the pointed N-terminal (i.e. PNT) domain which forms oligomer partners with itself as well as other transcription factors (e.g. FLI1) and is required for ETV6's transcriptional repressing activity; b) the central regulatory domain; and c) the C-terminal DNA-binding domain(PMID: 22578774). ETV6 is highly expressed in early hematopoietic progenitor cells(HPCs) and is essential for hematopoiesis in the bone marrow(PMID: 31248877).

实验方案

Product Specific Protocols
WB protocol for ETV6 antibody 67579-1-IgDownload protocol
IHC protocol for ETV6 antibody 67579-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols