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EVC2 Polyclonal antibody

EVC2 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Mouse, Human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 55367-1-AP

Print datasheet

Synonyms

Ellis van Creveld syndrome 2, EVC2, LBN, Limbin

验证数据展示

  • WB analysis using 55367-1-AP

    WB analysis using 55367-1-AP

    Mouse lung tissue were subjected to SDS PAGE followed by western blot with 55367-1-AP (EVC2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of human placenta using 55367-1-AP

    IHC staining of human placenta using 55367-1-AP

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 55367-1-AP (EVC2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected inmouse lung tissue
Positive IHC detected inhuman placenta tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

55367-1-AP targets EVC2 in WB, IHC, ELISA applications and shows reactivity with Mouse, Human samples.

Tested Applications WB, IHC, ELISA Application Description
Tested Reactivity Mouse, Human
Immunogen Peptide 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name Ellis van Creveld syndrome 2
Synonyms Ellis van Creveld syndrome 2, EVC2, LBN, Limbin
Calculated Molecular Weight 148 kDa
Observed Molecular Weight140 kDa
GenBank Accession NumberNM_147127
Gene Symbol EVC2
Gene ID (NCBI) 132884
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ86UK5
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

EVC2 (also known as LIMBIN) is close proximity in divergent orientation to EVC and mutations in both human genes lead to the chondrodysplasia Ellis-van Creveld (EvC) syndrome. Typical symptoms of EvC include shorter limbs and ribs, postaxial polydactyly, dysplastic nails, and cardiovascular defects (PMID: 28081373). Additionally, EvC syndrome has been characterized as a ciliopathy because the EVC and EVC2 proteins are intracellularly localized at the bottom of the primary cilium, where they form a heterotrimeric protein complex with SMO and transduce Hedgehog(Hh) signaling (PMID: 30410447). EVC2 is expressed in the heart, placenta, lung, liver, skeletal muscle, kidney, and pancreas. EVC2 has 3 isoforms with the molecular mass of 140-148 kDa.

实验方案

Product Specific Protocols
WB protocol for EVC2 antibody 55367-1-APDownload protocol
IHC protocol for EVC2 antibody 55367-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols