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验证数据展示

  • WB analysis using 68315-1-Ig (same clone as 68315-1-PBS)

    WB analysis using 68315-1-Ig (same clone as 68315-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68315-1-Ig (EXOSC10 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Lamin B1 Monoclonal antibody (HRP-66095) as loading control. This data was developed using the same antibody clone with 68315-1-PBS in a different storage buffer formulation.

  • IP experiment of HeLa using 68315-1-Ig (same clone as 68315-1-PBS)

    IP experiment of HeLa using 68315-1-Ig (same clone as 68315-1-PBS)

    IP result of anti-EXOSC10 (IP:68315-1-Ig, 4ug; Detection:68315-1-Ig 1:1000) with HeLa cells lysate 1720 ug. This data was developed using the same antibody clone with 68315-1-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 68315-1-Ig (same clone as 68315-1-PBS)

    IF Staining of HeLa using 68315-1-Ig (same clone as 68315-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using EXOSC10 antibody (68315-1-Ig, Clone: 1D6G9 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 68315-1-PBS in a different storage buffer formulation.

EXOSC10 Monoclonal antibody, PBS Only

EXOSC10 Monoclonal Antibody for WB, IF/ICC, IP, ELISA
Cat No. 68315-1-PBS

产品说明书

CloneNo. 1D6G9

宿主/亚型

Mouse / IgG1

种属反应性

Human

应用

WB, IF/ICC, IP, ELISA

别名

Autoantigen PM/Scl 2, EXOSC10, exosome component 10, p2, p3, p4, PM Scl, PM/Scl 100, PMSCL, PMSCL2, RRP6, Rrp6p

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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Planning an IHC experiment? We recommend our IHCeasy EXOSC10 Ready-To-Use IHC Kit. EXOSC10 primary antibody included.

产品信息

68315-1-PBS targets EXOSC10 in WB, IF/ICC, IP, ELISA applications and shows reactivity with Human samples.

经测试应用 WB, IF/ICC, IP, ELISA Application Description
经测试反应性 Human
免疫原 EXOSC10 fusion protein Ag28587 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 exosome component 10
别名 Autoantigen PM/Scl 2, EXOSC10, exosome component 10, p2, p3, p4, PM Scl, PM/Scl 100, PMSCL, PMSCL2, RRP6, Rrp6p
计算分子量 98 kDa
观测分子量98 kDa
GenBank蛋白编号BC039901
基因名称 EXOSC10
Gene ID (NCBI) 5394
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ01780
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

About 50% of patients with polymyositis/scleroderma (PM-Scl) overlap syndrome are reported to have autoantibodies to a neuclear/nucleolar particle termed PM-Scl. Exosome component 10 (EXOSC10), also named autoantigen PM/Scl 2, is the 100 kDa antigen component of PM-Scl and is recognized by most sera of PM-Scl paitents. EXOSC10 is strongly enriched in the nucleolus and a small amount has been found in cytoplasm supporting the existence of a nucleolar RNA exosome complex form. As a putative catalytic component of the RNA exosome complex which has 3'->5' exoribonuclease activity, EXOSC10 participates in a multitude of cellular RNA processing and degradation events.