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Conjugate
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验证数据展示

  • FC experiment of HepG2 using CL555-66099

    FC experiment of HepG2 using CL555-66099

    1X10^6 HepG2 cells were intracellularly stained with 0.8 ug CoraLite®555 Anti-Human EXOSC2 (CL555-66099, Clone:1G8B1) (red), or 0.8 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

CoraLite®555-conjugated EXOSC2 Monoclonal antibody

EXOSC2 Monoclonal Antibody for FC (Intra)
Cat No. CL555-66099

产品说明书

CloneNo. 1G8B1

宿主/亚型

Mouse / IgG2a

种属反应性

human

应用

FC (Intra)

别名

EXOSC2, Exosome complex component RRP4, Exosome component 2, hRrp4p, Ribosomal RNA-processing protein 4, RRP4, Rrp4p, 1G8B1

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite®555
规格: 

-/ -

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经过测试的应用

Positive FC (Intra) detected inHepG2 cells
Positive FC detected inHepG2 cells
For other applications, we recommend the unconjugated version of this antibody, 66099-1-Ig

推荐稀释比

应用推荐稀释比
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension
Flow Cytometry (FC)FC : 0.80 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL555-66099 targets EXOSC2 in FC (Intra) applications and shows reactivity with human samples.

经测试应用 FC (Intra) Application Description
经测试反应性 human
免疫原 EXOSC2 fusion protein Ag7003 种属同源性预测
宿主/亚型 Mouse / IgG2a
抗体类别 Monoclonal
产品类型 Antibody
全称 exosome component 2
别名 EXOSC2, Exosome complex component RRP4, Exosome component 2, hRrp4p, Ribosomal RNA-processing protein 4, RRP4, Rrp4p, 1G8B1
计算分子量 33 kDa
观测分子量 33 kDa
GenBank蛋白编号BC000747
基因名称 EXOSC2
Gene ID (NCBI) 23404
RRIDAB_3084623
偶联类型 CoraLite®555 Fluorescent Dye
最大激发/发射波长557 nm / 570 nm
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ13868
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

In the nucleus, the RNA exosome complex is involved in proper maturation of stable RNA species such as rRNA, snoRNA and snRNA, in the elimination of RNA processing by-products and non-coding 'pervasive' transcripts, such as antisense RNA species and promoter-upstream transcripts (PROMPTs), and of mRNAs with processing defects, thereby limiting or excluding their export to the cytoplasm. In the cytoplasm, the RNA exosome complex is involved in general mRNA turnover and specifically degrades inherently unstable mRNAs containing AU-rich elements (AREs) within their 3' untranslated regions, and in RNA surveillance pathways, preventing translation of aberrant mRNAs [PMID:15346807]. EXOSC2 is a non-catalytic component of the RNA exosome complex that has 3'->5' exoribonuclease activity and involves in a multitude of cellular RNA processing and degradation events [PMID: 17545563].

实验方案

Product Specific Protocols
FC protocol for CL555 EXOSC2 antibody CL555-66099Download protocol
Standard Protocols
Click here to view our Standard Protocols