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  • Featured Product
  • KD/KO Validated

EXOSC9 Monoclonal antibody, PBS Only

EXOSC9 Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

Human, Mouse, Rat

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

3F4G1

Cat no : 67636-1-PBS

Print datasheet

Synonyms

Autoantigen PM/Scl 1, EXOSC9, exosome component 9, p5, p6, PM/Scl 75, PMSCL1, RRP45, Rrp45p

验证数据展示

  • WB analysis using 67636-1-Ig (same clone as 67636-1-PBS)

    WB analysis using 67636-1-Ig (same clone as 67636-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67636-1-Ig (EXOSC9 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 67636-1-PBS in a different storage buffer formulation.

  • WB analysis of HeLa using 67636-1-Ig (same clone as 67636-1-PBS)

    WB analysis of HeLa using 67636-1-Ig (same clone as 67636-1-PBS)

    WB result of EXOSC9 antibody (67636-1-Ig; 1:50000; incubated at room temperature for 1.5 hours) with sh-Control and sh-EXOSC9 transfected HeLa cells. This data was developed using the same antibody clone with 67636-1-PBS in a different storage buffer formulation.

  • IHC staining of human breast cancer using 67636-1-Ig (same clone as 67636-1-PBS)

    IHC staining of human breast cancer using 67636-1-Ig (same clone as 67636-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67636-1-Ig (EXOSC9 antibody) at dilution of 1:1500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67636-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with EXOSC9 antibody included
  • IF Staining of HeLa using 67636-1-Ig (same clone as 67636-1-PBS)

    IF Staining of HeLa using 67636-1-Ig (same clone as 67636-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using EXOSC9 antibody (67636-1-Ig, Clone: 3F4G1 ) at dilution of 1:2000 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L), CL594-phalloidin (red). This data was developed using the same antibody clone with 67636-1-PBS in a different storage buffer formulation.

Planning an IHC experiment? We recommend our IHCeasy EXOSC9 Ready-To-Use IHC Kit. EXOSC9 primary antibody included.

产品信息

67636-1-PBS targets EXOSC9 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with Human, Mouse, Rat samples.

Tested Applications WB, IHC, IF/ICC, Indirect ELISA Application Description
Tested Reactivity Human, Mouse, Rat
Immunogen EXOSC9 fusion protein Ag19783 种属同源性预测
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Full Name exosome component 9
Synonyms Autoantigen PM/Scl 1, EXOSC9, exosome component 9, p5, p6, PM/Scl 75, PMSCL1, RRP45, Rrp45p
Calculated Molecular Weight 456 aa, 51 kDa
Observed Molecular Weight 65-70 kDa
GenBank Accession NumberBC142978
Gene Symbol EXOSC9
Gene ID (NCBI) 5393
RRIDAB_2882837
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ06265
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

EXOSC9, also named as PMSCL1 and PM/Scl-75, is a key subunit of the exosome complex. EXOSC9 maintains self-renewal in a cell-autonomous manner. EXOSC9 has 4 isoforms with MW 49 kDa, 51 kDa, 39 kDa and 41 kDa. It's polymyositis/scleroderma auto-antigen 75 kDa. The MW of EXOSC9 is migrated to 60-75 kDa for modification in WB detection.