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验证数据展示

  • WB analysis using 67636-1-Ig

    WB analysis using 67636-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67636-1-Ig (EXOSC9 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.

  • WB analysis of HeLa using 67636-1-Ig

    WB analysis of HeLa using 67636-1-Ig

    WB result of EXOSC9 antibody (67636-1-Ig; 1:50000; incubated at room temperature for 1.5 hours) with sh-Control and sh-EXOSC9 transfected HeLa cells.

  • IHC staining of human breast cancer using 67636-1-Ig

    IHC staining of human breast cancer using 67636-1-Ig

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67636-1-Ig (EXOSC9 antibody) at dilution of 1:1500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with EXOSC9 antibody included
  • IF Staining of HeLa using 67636-1-Ig

    IF Staining of HeLa using 67636-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using EXOSC9 antibody (67636-1-Ig, Clone: 3F4G1 ) at dilution of 1:2000 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L), CL594-phalloidin (red).

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  • KD/KO验证

EXOSC9 Monoclonal antibody

EXOSC9 Monoclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 67636-1-Ig

产品说明书

CloneNo. 3F4G1

宿主/亚型

Mouse / IgG2a

种属反应性

Human, Mouse, Rat

应用

WB, IHC, IF/ICC, ELISA

别名

Autoantigen PM/Scl 1, EXOSC9, exosome component 9, p5, p6, PM/Scl 75, PMSCL1, RRP45, Rrp45p

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inU2OS cells, HeLa cells, A549 cells, MCF-7 cells, HepG2 cells, K-562 cells, Jurkat cells, HSC-T6 cells, NIH/3T3 cells
Positive IHC detected inhuman breast cancer tissue, mouse kidney tissue, rat kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy EXOSC9 Ready-To-Use IHC Kit. EXOSC9 primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 and CoraLite®594 conjugated versions of this antibody.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:750-1:3000
Immunofluorescence (IF)/ICCIF/ICC : 1:1000-1:4000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

67636-1-Ig targets EXOSC9 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with Human, Mouse, Rat samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
经测试反应性 Human, Mouse, Rat
免疫原 EXOSC9 fusion protein Ag19783 种属同源性预测
宿主/亚型 Mouse / IgG2a
抗体类别 Monoclonal
产品类型 Antibody
全称 exosome component 9
别名 Autoantigen PM/Scl 1, EXOSC9, exosome component 9, p5, p6, PM/Scl 75, PMSCL1, RRP45, Rrp45p
计算分子量 456 aa, 51 kDa
观测分子量 65-70 kDa
GenBank蛋白编号BC142978
基因名称 EXOSC9
Gene ID (NCBI) 5393
RRIDAB_2882837
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ06265
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

EXOSC9, also named as PMSCL1 and PM/Scl-75, is a key subunit of the exosome complex. EXOSC9 maintains self-renewal in a cell-autonomous manner. EXOSC9 has 4 isoforms with MW 49 kDa, 51 kDa, 39 kDa and 41 kDa. It's polymyositis/scleroderma auto-antigen 75 kDa. The MW of EXOSC9 is migrated to 60-75 kDa for modification in WB detection.

实验方案

Product Specific Protocols
WB protocol for EXOSC9 antibody 67636-1-IgDownload protocol
IHC protocol for EXOSC9 antibody 67636-1-IgDownload protocol
IF protocol for EXOSC9 antibody 67636-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols