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验证数据展示

  • FC experiment of HeLa using CL488-67636

    FC experiment of HeLa using CL488-67636

    1X10^6 HeLa cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human EXOSC9 (CL488-67636, Clone:3F4G1) (red), or 0.4 ug Mouse IgG2a Isotype Control (CL488-66360-2, Clone: K11A1B2A2) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

  • IF Staining of HeLa using CL488-67636

    IF Staining of HeLa using CL488-67636

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CoraLite® Plus 488 EXOSC9 antibody (CL488-67636, Clone: 3F4G1 ) at dilution of 1:200.

  • Featured Product
  • KD/KO验证

CoraLite® Plus 488-conjugated EXOSC9 Monoclonal antibody

EXOSC9 Monoclonal Antibody for IF/ICC, FC (Intra)
Cat No. CL488-67636

产品说明书

CloneNo. 3F4G1

宿主/亚型

Mouse / IgG2a

种属反应性

human, mouse, rat

应用

IF/ICC, FC (Intra)

别名

PMSCL1, PM/Scl-75, P75 polymyositis-scleroderma overlap syndrome-associated autoantigen, Exosome complex component RRP45, 3F4G1

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite® Plus 488
规格: 

-/ -

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经过测试的应用

Positive IF/ICC detected inHeLa cells
Positive FC (Intra) detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy EXOSC9 Ready-To-Use IHC Kit. EXOSC9 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 67636-1-Ig

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-67636 targets EXOSC9 in IF/ICC, FC (Intra) applications and shows reactivity with human, mouse, rat samples.

经测试应用 IF/ICC, FC (Intra) Application Description
经测试反应性 human, mouse, rat
免疫原 EXOSC9 fusion protein Ag19783 种属同源性预测
宿主/亚型 Mouse / IgG2a
抗体类别 Monoclonal
产品类型 Antibody
全称 exosome component 9
别名 PMSCL1, PM/Scl-75, P75 polymyositis-scleroderma overlap syndrome-associated autoantigen, Exosome complex component RRP45, 3F4G1
计算分子量 456 aa, 51 kDa
观测分子量 65-70 kDa
GenBank蛋白编号BC142978
基因名称 EXOSC9
Gene ID (NCBI) 5393
RRIDAB_2934555
偶联类型 CoraLite® Plus 488 Fluorescent Dye
最大激发/发射波长493 nm / 522 nm
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ06265
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

EXOSC9, also named as PMSCL1 and PM/Scl-75, is a key subunit of the exosome complex. EXOSC9 maintains self-renewal in a cell-autonomous manner. EXOSC9 has 4 isoforms with MW 49 kDa, 51 kDa, 39 kDa and 41 kDa. It's polymyositis/scleroderma auto-antigen 75 kDa. The MW of EXOSC9 is migrated to 60-75 kDa for modification in WB detection.

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 EXOSC9 antibody CL488-67636Download protocol
FC protocol for CL Plus 488 EXOSC9 antibody CL488-67636Download protocol
Standard Protocols
Click here to view our Standard Protocols