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  • Featured Product
  • KD/KO Validated

CoraLite®594-conjugated EXOSC9 Monoclonal antibody

EXOSC9 Monoclonal Antibody for IF/ICC, FC (Intra)

Host / Isotype

Mouse / IgG2a

Reactivity

human, mouse, rat

Applications

IF/ICC, FC (Intra)

Conjugate

CoraLite®594 Fluorescent Dye

CloneNo.

3F4G1

Cat no : CL594-67636

Print datasheet

Synonyms

PMSCL1, PM/Scl-75, P75 polymyositis-scleroderma overlap syndrome-associated autoantigen, Exosome complex component RRP45, 3F4G1

验证数据展示

  • FC experiment of HeLa using CL594-67636

    FC experiment of HeLa using CL594-67636

    1X10^6 HeLa cells were intracellularly stained with 0.4 ug CoraLite®594 Anti-Human EXOSC9 (CL594-67636, Clone:3F4G1) (red), or 0.4 ug Isotype Control. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

  • IF Staining of HeLa using CL594-67636

    IF Staining of HeLa using CL594-67636

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CoraLite®594 EXOSC9 antibody (CL594-67636, Clone: 3F4G1 ) at dilution of 1:400.

经过测试的应用

Positive IF/ICC detected inHeLa cells
Positive FC (Intra) detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy EXOSC9 Ready-To-Use IHC Kit. EXOSC9 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 67636-1-Ig

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL594-67636 targets EXOSC9 in IF/ICC, FC (Intra) applications and shows reactivity with human, mouse, rat samples.

Tested Applications IF/ICC, FC (Intra) Application Description
Tested Reactivity human, mouse, rat
Immunogen EXOSC9 fusion protein Ag19783 种属同源性预测
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Full Name exosome component 9
Synonyms PMSCL1, PM/Scl-75, P75 polymyositis-scleroderma overlap syndrome-associated autoantigen, Exosome complex component RRP45, 3F4G1
Calculated Molecular Weight 456 aa, 51 kDa
Observed Molecular Weight 65-70 kDa
GenBank Accession NumberBC142978
Gene Symbol EXOSC9
Gene ID (NCBI) 5393
Conjugate CoraLite®594 Fluorescent Dye
Excitation/Emission Maxima Wavelengths588 nm / 604 nm
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ06265
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

EXOSC9, also named as PMSCL1 and PM/Scl-75, is a key subunit of the exosome complex. EXOSC9 maintains self-renewal in a cell-autonomous manner. EXOSC9 has 4 isoforms with MW 49 kDa, 51 kDa, 39 kDa and 41 kDa. It's polymyositis/scleroderma auto-antigen 75 kDa. The MW of EXOSC9 is migrated to 60-75 kDa for modification in WB detection.

实验方案

Product Specific Protocols
IF protocol for CL594 EXOSC9 antibody CL594-67636Download protocol
FC protocol for CL594 EXOSC9 antibody CL594-67636Download protocol
Standard Protocols
Click here to view our Standard Protocols