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验证数据展示

  • FC experiment of HeLa using CL488-23333

    FC experiment of HeLa using CL488-23333

    1X10^6 HeLa cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human Eg5 (CL488-23333) (red), or 0.4 ug Isotype Control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • IF Staining of HepG2 using CL488-23333

    IF Staining of HepG2 using CL488-23333

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CoraLite® Plus 488 Eg5 antibody (CL488-23333) at dilution of 1:200, CL594-phalloidin (red).

  • Featured Product
  • KD/KO Validated

CoraLite® Plus 488-conjugated Eg5 Polyclonal antibody

Eg5 Polyclonal Antibody for IF/ICC, FC (Intra)
Cat No. CL488-23333

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC, FC (Intra)

Synonyms

KIF11, KNSL1, Kinesin-related motor protein Eg5, Kinesin-like spindle protein HKSP, Kinesin-like protein KIF11

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  CoraLite® Plus 488
Size: 

-/ -

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经过测试的应用

Positive IF/ICC detected inHepG2 cells
Positive FC (Intra) detected inHeLa cells
For other applications, we recommend the unconjugated version of this antibody, 23333-1-AP

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-23333 targets Eg5 in IF/ICC, FC (Intra) applications and shows reactivity with human samples.

Tested Applications IF/ICC, FC (Intra) Application Description
Tested Reactivity human
Immunogen Eg5 fusion protein Ag18695 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name kinesin family member 11
Synonyms KIF11, KNSL1, Kinesin-related motor protein Eg5, Kinesin-like spindle protein HKSP, Kinesin-like protein KIF11
Calculated Molecular Weight 1056 aa, 119 kDa
Observed Molecular Weight 120-130 kDa
GenBank Accession NumberBC136474
Gene Symbol Eg5
Gene ID (NCBI) 3832
RRIDAB_3084073
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP52732
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Eg5 is a plus-end-directed microtubule motor encoded by KIF11 gene. It is an essential mitotic kinesin that plays a pivotal role in the formation and maintenance of bipolar spindle during early prometaphase. Eg5 has been implicated in tumourigenesis, and its overexpression has been found in various cancer tissues.

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 Eg5 antibody CL488-23333Download protocol
FC protocol for CL Plus 488 Eg5 antibody CL488-23333Download protocol
Standard Protocols
Click here to view our Standard Protocols