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验证数据展示

  • FC experiment of HeLa using CL488-23333

    FC experiment of HeLa using CL488-23333

    1X10^6 HeLa cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human Eg5 (CL488-23333) (red), or 0.4 ug Isotype Control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • IF Staining of HepG2 using CL488-23333

    IF Staining of HepG2 using CL488-23333

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CoraLite® Plus 488 Eg5 antibody (CL488-23333) at dilution of 1:200, CL594-phalloidin (red).

  • Featured Product
  • KD/KO验证

CoraLite® Plus 488-conjugated Eg5 Polyclonal antibody

Eg5 Polyclonal Antibody for IF/ICC, FC (Intra)
Cat No. CL488-23333

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

IF/ICC, FC (Intra)

别名

KIF11, KNSL1, Kinesin-related motor protein Eg5, Kinesin-like spindle protein HKSP, Kinesin-like protein KIF11

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite® Plus 488
规格: 

-/ -

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经过测试的应用

Positive IF/ICC detected inHepG2 cells
Positive FC (Intra) detected inHeLa cells
For other applications, we recommend the unconjugated version of this antibody, 23333-1-AP

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-23333 targets Eg5 in IF/ICC, FC (Intra) applications and shows reactivity with human samples.

经测试应用 IF/ICC, FC (Intra) Application Description
经测试反应性 human
免疫原 Eg5 fusion protein Ag18695 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 kinesin family member 11
别名 KIF11, KNSL1, Kinesin-related motor protein Eg5, Kinesin-like spindle protein HKSP, Kinesin-like protein KIF11
计算分子量 1056 aa, 119 kDa
观测分子量 120-130 kDa
GenBank蛋白编号BC136474
基因名称 Eg5
Gene ID (NCBI) 3832
RRIDAB_3084073
偶联类型 CoraLite® Plus 488 Fluorescent Dye
最大激发/发射波长493 nm / 522 nm
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP52732
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Eg5 is a plus-end-directed microtubule motor encoded by KIF11 gene. It is an essential mitotic kinesin that plays a pivotal role in the formation and maintenance of bipolar spindle during early prometaphase. Eg5 has been implicated in tumourigenesis, and its overexpression has been found in various cancer tissues.

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 Eg5 antibody CL488-23333Download protocol
FC protocol for CL Plus 488 Eg5 antibody CL488-23333Download protocol
Standard Protocols
Click here to view our Standard Protocols