CoraLite® Plus 488-conjugated Endoglin/CD105 Monoclonal antibody
Endoglin/CD105 Monoclonal Antibody for IF-P
Host / Isotype
Mouse / IgG2b
Reactivity
Human
Applications
IF-P
Conjugate
CoraLite® Plus 488 Fluorescent Dye
CloneNo.
2D5E8
验证数据展示
经过测试的应用
Positive IF-P detected in | Human liver cancer tissue |
Planning an IHC experiment? We recommend our IHCeasy CD105 Ready-To-Use IHC Kit. CD105 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 67075-1-Ig
推荐稀释比
Application | Dilution |
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Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
CL488-67075 targets Endoglin/CD105 in IF-P applications and shows reactivity with Human samples.
Tested Applications | IF-P Application Description |
Tested Reactivity | Human |
Immunogen | Endoglin/CD105 fusion protein Ag27837 种属同源性预测 |
Host / Isotype | Mouse / IgG2b |
Class | Monoclonal |
Type | Antibody |
Full Name | endoglin |
Synonyms | CD105, END, Endoglin, ENG, HHT1, ORW, ORW1, 2D5E8 |
Calculated Molecular Weight | 71 kDa |
Observed Molecular Weight | 95-100 kDa |
GenBank Accession Number | BC014271 |
Gene Symbol | CD105 |
Gene ID (NCBI) | 2022 |
RRID | AB_2919417 |
Conjugate | CoraLite® Plus 488 Fluorescent Dye |
Excitation/Emission Maxima Wavelengths | 493 nm / 522 nm |
Form | Liquid |
Purification Method | Protein A purification |
UNIPROT ID | P17813 |
Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Endoglin (ENG, CD105) is a homodimeric cell membrane glycoprotein of 180 kDa, composed of disulphide-linked subunits of 95 kDa. Endoglin is a proliferation-associated and hypoxia-inducible protein mainly expressed on vascular endothelial cells. It acts as an accessory receptor for transforming growth factor beta (TFG-β) and is involved in vascular development and remodelling. The important role of Endoglin in angiogenesis and in tumor progression makes it an ideal target for antiangiogenic therapy and a good marker for tumor prognosis. (PMID: 1326540; 14528280; 21737653; 12773481)
实验方案
Product Specific Protocols | |
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IF protocol for CL Plus 488 Endoglin/CD105 antibody CL488-67075 | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |