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CoraLite® Plus 488 Anti-Human Endoglin/CD105 Rabbit Recombinant Antibody

Endoglin/CD105 Uni-rAbTM Recombinant Antibody for FC

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

FC

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

230047E5

Cat no : CL488-98013

Print datasheet

Synonyms

CD105, 230047E5, HHT1, ENG, Endoglin

验证数据展示

  • FC experiment of human PBMCs using CL488-98013

    FC experiment of human PBMCs using CL488-98013

    1x10^6 human PBMCs were surface stained with 5 ul CoraLite® Plus 488 Anti-Human Endoglin/CD105 Rabbit Recombinant Antibody (CL488-98013, Clone:230047E5), or Isotype Control. Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed.

  • FC experiment of human PBMCs using CL488-98013

    FC experiment of human PBMCs using CL488-98013

    1x10^6 human PBMCs were surface stained with 5 ul CoraLite® Plus 488 Anti-Human Endoglin/CD105 Rabbit Recombinant Antibody (CL488-98013, Clone:230047E5)(red), or Isotype Control (blue). Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed.

经过测试的应用

Positive FC detected inhuman PBMCs
Planning an IHC experiment? We recommend our IHCeasy CD105 Ready-To-Use IHC Kit. CD105 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 98013-1-PBS

推荐稀释比

ApplicationDilution
This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 ul per 10^6 cells in a 100 µl suspension or 5 ul per 100 µl of whole blood.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-98013 targets Endoglin/CD105 in FC applications and shows reactivity with human samples.

Tested Applications FC Application Description
Tested Reactivity human
Immunogen Recombinant protein 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name endoglin
Synonyms CD105, 230047E5, HHT1, ENG, Endoglin
Calculated Molecular Weight 71 kDa
GenBank Accession NumberBC014271
Gene Symbol CD105
Gene ID (NCBI) 2022
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP17813
Storage Buffer PBS with 0.09% sodium azide and 0.5% BSA.
Storage ConditionsStore at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

背景介绍

Endoglin (ENG, CD105) is a homodimeric cell membrane glycoprotein of 180 kDa, composed of disulphide-linked subunits of 90-95 kDa. Endoglin is a proliferation-associated and hypoxia-inducible protein mainly expressed on vascular endothelial cells. It acts as an accessory receptor for transforming growth factor beta (TFG-β) and is involved in vascular development and remodeling. The important role of Endoglin in angiogenesis and in tumor progression makes it an ideal target for antiangiogenic therapy and a good marker for tumor prognosis. (PMID: 1326540; 14528280; 21737653; 12773481)

实验方案

Product Specific Protocols
FC protocol for CL Plus 488 Endoglin/CD105 antibody CL488-98013Download protocol
Standard Protocols
Click here to view our Standard Protocols