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  • Featured Product
  • KD/KO Validated

CoraLite® Plus 488-conjugated Ezrin Polyclonal antibody

Ezrin Polyclonal Antibody for FC (Intra)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

FC (Intra)

Conjugate

CoraLite® Plus 488 Fluorescent Dye

Cat no : CL488-26056

Print datasheet

Synonyms

CVIL, CVL, Cytovillin, DKFZp762H157, EZR, ezrin, FLJ26216, p81, VIL2, Villin 2

验证数据展示

  • FC experiment of HeLa using CL488-26056

    FC experiment of HeLa using CL488-26056

    1X10^6 HeLa cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human Ezrin (CL488-26056) (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

经过测试的应用

Positive FC (Intra) detected inHeLa cells
Positive FC detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy Ezrin Ready-To-Use IHC Kit. Ezrin primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 26056-1-AP

推荐稀释比

ApplicationDilution
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-26056 targets Ezrin in FC (Intra) applications and shows reactivity with human, mouse samples.

Tested Applications FC (Intra) Application Description
Tested Reactivity human, mouse
Immunogen Ezrin fusion protein Ag23530 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name ezrin
Synonyms CVIL, CVL, Cytovillin, DKFZp762H157, EZR, ezrin, FLJ26216, p81, VIL2, Villin 2
Calculated Molecular Weight 69 kDa
Observed Molecular Weight 70-75 kDa
GenBank Accession NumberBC013903
Gene Symbol Ezrin
Gene ID (NCBI) 7430
ENSEMBL Gene IDENSG00000092820
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP15311
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
FC protocol for CL Plus 488 Ezrin antibody CL488-26056Download protocol
Standard Protocols
Click here to view our Standard Protocols