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验证数据展示

  • IF Staining of HeLa using CL488-26056

    IF Staining of HeLa using CL488-26056

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using CoraLite® Plus 488 Ezrin antibody (CL488-26056) at dilution of 1:600.

  • FC experiment of HeLa using CL488-26056

    FC experiment of HeLa using CL488-26056

    1X10^6 HeLa cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human Ezrin (CL488-26056) (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • Featured Product
  • KD/KO Validated

CoraLite® Plus 488-conjugated Ezrin Polyclonal antibody

Ezrin Polyclonal Antibody for IF/ICC, FC (Intra)
Cat No. CL488-26056

Print datasheet

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

IF/ICC, FC (Intra)

Synonyms

Villin-2, VIL2, EZR

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  CoraLite® Plus 488
Size: 

-/ -

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经过测试的应用

Positive IF/ICC detected inHeLa cells
Positive FC (Intra) detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy Ezrin Ready-To-Use IHC Kit. Ezrin primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 26056-1-AP

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:300-1:1200
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-26056 targets Ezrin in IF/ICC, FC (Intra) applications and shows reactivity with human, mouse samples.

Tested Applications IF/ICC, FC (Intra) Application Description
Tested Reactivity human, mouse
Immunogen Ezrin fusion protein Ag23530 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name ezrin
Synonyms Villin-2, VIL2, EZR
Calculated Molecular Weight 69 kDa
Observed Molecular Weight 70-75 kDa
GenBank Accession NumberBC013903
Gene Symbol Ezrin
Gene ID (NCBI) 7430
ENSEMBL Gene IDENSG00000092820
RRIDAB_3084096
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP15311
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 Ezrin antibody CL488-26056Download protocol
FC protocol for CL Plus 488 Ezrin antibody CL488-26056Download protocol
Standard Protocols
Click here to view our Standard Protocols