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FABP1 Monoclonal antibody, PBS Only

FABP1 Monoclonal Antibody for WB, FC (Intra), Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat, pig

Applications

WB, FC (Intra), Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1B6C8

Cat no : 68227-1-PBS

Print datasheet

Synonyms

liver FABP, L-FABP, Fatty acid-binding protein, liver, Fatty acid-binding protein 1, Fatty acid binding protein 1

验证数据展示

  • WB analysis using 68227-1-Ig (same clone as 68227-1-PBS)

    WB analysis using 68227-1-Ig (same clone as 68227-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68227-1-Ig (FABP1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68227-1-PBS in a different storage buffer formulation.

  • WB analysis of pig liver using 68227-1-Ig (same clone as 68227-1-PBS)

    WB analysis of pig liver using 68227-1-Ig (same clone as 68227-1-PBS)

    pig liver tissue were subjected to SDS PAGE followed by western blot with 68227-1-Ig (FABP1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68227-1-PBS in a different storage buffer formulation.

  • FC experiment of HepG2 using 68227-1-Ig (same clone as 68227-1-PBS)

    FC experiment of HepG2 using 68227-1-Ig (same clone as 68227-1-PBS)

    1x10^6 HepG2 cells were intracellularly stained with 0.8 ug FABP1 Monoclonal antibody (68227-1-Ig, Clone:1B6C8) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1)(red), or 0.8 ug Mouse IgG1 isotype control Mouse McAb (66360-1-Ig, Clone: 1F8D3) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 68227-1-PBS in a different storage buffer formulation.

产品信息

68227-1-PBS targets FABP1 in WB, FC (Intra), Indirect ELISA applications and shows reactivity with human, mouse, rat, pig samples.

Tested Applications WB, FC (Intra), Indirect ELISA Application Description
Tested Reactivity human, mouse, rat, pig
Immunogen FABP1 fusion protein Ag32836 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name fatty acid binding protein 1, liver
Synonyms liver FABP, L-FABP, Fatty acid-binding protein, liver, Fatty acid-binding protein 1, Fatty acid binding protein 1
Calculated Molecular Weight 127 aa, 14 kDa
Observed Molecular Weight 14 kDa
GenBank Accession NumberBC032801
Gene Symbol FABP1
Gene ID (NCBI) 2168
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDP07148
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

FABP1, liver fatty acid-binding protein, is abundant in cytoplasm that regulates lipid transport and metabolism. It plays a role in lipoprotein-mediated cholesterol uptake in hepatocytes (PMID:25732850). FABP1 is mainly expressed in hepatocytes, enterocytes and to a lesser degree in renal tubular cells, associated with liver injury (PMID: 15653098). Levels of FABP1 was elevated in patients with hepatocyte injury secondary to alcohol or drug toxicity (PMID: 14563446).