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验证数据展示

  • IF Staining of HeLa using CL488-12348

    IF Staining of HeLa using CL488-12348

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using CoraLite® Plus 488 FABP5 antibody (CL488-12348) at dilution of 1:200.

  • FC experiment of HeLa using CL488-12348

    FC experiment of HeLa using CL488-12348

    1X10^6 HeLa cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human FABP5 (CL488-12348) (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • Featured Product
  • KD/KO验证

CoraLite® Plus 488-conjugated FABP5 Polyclonal antibody

FABP5 Polyclonal Antibody for IF/ICC, FC (Intra)
Cat No. CL488-12348

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

IF/ICC, FC (Intra)

别名

PAFABP, Fatty acid-binding protein, epidermal, Fatty acid-binding protein 5, Fatty acid binding protein 5, Epidermal-type fatty acid-binding protein

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite® Plus 488
规格: 

-/ -

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经过测试的应用

Positive IF/ICC detected inHeLa cells
Positive FC (Intra) detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy FABP5 Ready-To-Use IHC Kit. FABP5 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 12348-1-AP

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-12348 targets FABP5 in IF/ICC, FC (Intra) applications and shows reactivity with human, mouse, rat samples.

经测试应用 IF/ICC, FC (Intra) Application Description
经测试反应性 human, mouse, rat
免疫原 FABP5 fusion protein Ag3005 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 fatty acid binding protein 5 (psoriasis-associated)
别名 PAFABP, Fatty acid-binding protein, epidermal, Fatty acid-binding protein 5, Fatty acid binding protein 5, Epidermal-type fatty acid-binding protein
计算分子量 135 aa, 15 kDa
观测分子量 15 kDa
GenBank蛋白编号BC019385
基因名称 FABP5
Gene ID (NCBI) 2171
RRIDAB_2919050
偶联类型 CoraLite® Plus 488 Fluorescent Dye
最大激发/发射波长493 nm / 522 nm
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ01469
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

FABP5, also named as PA-FABP and E-FABP, belongs to the calycin superfamily and Fatty-acid binding protein (FABP) family. It is high specificity for fatty acids. FABP5 is highest affinity for C18 chain length. It may be involved in keratinocyte differentiation. FABP5 is a fatty acid-binding protein and is expressed in epidermis and endothelial cells of the microvasculature of different organs. FABP5 has also been identified as a tumor-associated antigen, which is highly expressed in various cancers. FABP5 was detected in the sera of HNSCC patients with early stage cancer. Antibodies specific for FABP5 were significantly increased in a substantial amount in patients, suggesting that FABP5 may be a potential diagnostic biomarker for HNSCC. FABP5 may serve as a biomarker for HNSCC.(PMID:19602232)

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 FABP5 antibody CL488-12348Download protocol
FC protocol for CL Plus 488 FABP5 antibody CL488-12348Download protocol
Standard Protocols
Click here to view our Standard Protocols