验证数据展示
经过测试的应用
| Positive WB detected in | HEK-293 cells, HepG2 cells, HeLa cells, mouse brain tissue, rat brain tissue, K-562 cells, MCF-7 cells, SH-SY5Y cells, U2OS cells |
| Positive IP detected in | mouse brain tissue |
| Positive IHC detected in | human breast cancer tissue, mouse brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:8000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
16251-1-AP targets FAF2 in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IP, IHC, ELISA Application Description |
| 文献引用应用 | WB, IF, IP |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, rat, bovine |
| 免疫原 | FAF2 fusion protein Ag9236 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | Fas associated factor family member 2 |
| 别名 | ETEA, FAF2, FAS associated factor 2, KIAA0887, Protein ETEA, UBXD8, UBXN3B |
| 计算分子量 | 445 aa, 53 kDa |
| 观测分子量 | 53 kDa |
| GenBank蛋白编号 | BC014001 |
| 基因名称 | FAF2 |
| Gene ID (NCBI) | 23197 |
| RRID | AB_2262469 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q96CS3 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Protein ETEA (FAF2, or UBXD8) is a homolog to Fas-associated factor 1 (FAF1), which is involved in Fas-mediated apoptosis. ETEA protein directly interacts with and negatively regulates neurofibromin. ETEA contains both UBA and UBX domains and overexpression of ETEA downregulates neurofibromin in human cells. It may play a role in the translocation of terminally misfolded proteins from the endoplasmic reticulum lumen to the cytoplasm and their degradation by the proteasome. ETEA is highly expressed in peripheral blood of patients with atopic dermatitis (AD) compared to normal individuals, and may regulate the resistance to apoptosis that is observed in T cells and eosinophils of AD patients.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for FAF2 antibody 16251-1-AP | Download protocol |
| IHC protocol for FAF2 antibody 16251-1-AP | Download protocol |
| IP protocol for FAF2 antibody 16251-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Science Ubiquitination of G3BP1 mediates stress granule disassembly in a context-specific manner. | ||
Cell Rep hnRNPA2B1 represses the disassembly of arsenite-induced stress granules and is essential for male fertility | ||
Elife USP13 antagonizes gp78 to maintain functionality of a chaperone in ER-associated degradation. | ||
Elife Interaction mapping of endoplasmic reticulum ubiquitin ligases identifies modulators of innate immune signalling. | ||
J Biol Chem A Ubiquitin-like Domain Recruits an Oligomeric Chaperone to a Retrotranslocation Complex in Endoplasmic Reticulum-associated Degradation. | ||
J Biol Chem Characterization of the deubiquitinating activity of USP19 and its role in endoplasmic reticulum-associated degradation. |
