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验证数据展示

  • WB analysis using 83933-1-RR (same clone as 83933-1-PBS)

    WB analysis using 83933-1-RR (same clone as 83933-1-PBS)

    Non-treated and pervanadate treated HeLa cells were subjected to SDS PAGE followed by western blot with 83933-1-RR (Phospho-FAK (Tyr397) antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with Beta Actin (66009-1-Ig) antibody as a loading control. This data was developed using the same antibody clone with 83933-1-PBS in a different storage buffer formulation.

  • IF Staining of A549 using 83933-1-RR (same clone as 83933-1-PBS)

    IF Staining of A549 using 83933-1-RR (same clone as 83933-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed A549 cells using Phospho-FAK (Tyr397) antibody (83933-1-RR, Clone: 240630F8 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 83933-1-PBS in a different storage buffer formulation.

Phospho-FAK (Tyr397) Recombinant antibody, PBS Only

Phospho-FAK (Tyr397) Uni-rAbTM Recombinant Antibody for WB, IF/ICC, ELISA
Cat No. 83933-1-PBS

产品说明书

CloneNo. 240630F8

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IF/ICC, ELISA

别名

FAK (Tyr397), p FAK, p FAK (Tyr397), p FAK Tyr397, p-FAK

缓冲液配方:  PBS Only
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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Planning an IHC experiment? We recommend our IHCeasy FAK Ready-To-Use IHC Kit. FAK primary antibody included.

产品信息

83933-1-PBS targets Phospho-FAK (Tyr397) in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IF/ICC, ELISA Application Description
经测试反应性 human
免疫原 Peptide 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 PTK2 protein tyrosine kinase 2
别名 FAK (Tyr397), p FAK, p FAK (Tyr397), p FAK Tyr397, p-FAK
计算分子量 1052 aa, 119 kDa
观测分子量 110 kDa
GenBank蛋白编号BC028733
基因名称 FAK
Gene ID (NCBI) 5747
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purfication
UNIPROT IDQ05397
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

FAK (Focal adhesion kinase 1) is also named as FAK1, FADK, pp125FAK, FAK and belongs to the protein kinase superfamily. It is a critical tyrosine kinase that modulates cell adhesion, migration, proliferation and survival in response to extracellular signals (PMID:19664602). It also acts as a pivotal signal 'integrator', controlling and coordinating cellular responses that include cell migration, survival, proliferation and, epithelial tissue repair after DNA damage (PMID:20966971). This protein has some isoforms produced by alternative promoter usage and alternative splicing, and the range of the molecular weights are 100-120 kDa and 40-60 kDa.