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Phospho-FAK (Tyr397) Recombinant antibody, PBS Only

Phospho-FAK (Tyr397) Uni-rAbTM Recombinant Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

240630F8

Cat no : 83933-1-PBS

Print datasheet

Synonyms

FAK, PTK2, Focal adhesion kinase 1, FAK1, FADK 1

验证数据展示

  • WB analysis using 83933-1-RR (same clone as 83933-1-PBS)

    WB analysis using 83933-1-RR (same clone as 83933-1-PBS)

    Non-treated and pervanadate treated HeLa cells were subjected to SDS PAGE followed by western blot with 83933-1-RR (FAK antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with Beta Actin (66009-1-Ig) antibody as a loading control. This data was developed using the same antibody clone with 83933-1-PBS in a different storage buffer formulation.

  • IF Staining of A549 using 83933-1-RR (same clone as 83933-1-PBS)

    IF Staining of A549 using 83933-1-RR (same clone as 83933-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed A549 cells using FAK antibody (83933-1-RR, Clone: 240630F8 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 83933-1-PBS in a different storage buffer formulation.

Planning an IHC experiment? We recommend our IHCeasy FAK Ready-To-Use IHC Kit. FAK primary antibody included.

产品信息

83933-1-PBS targets Phospho-FAK (Tyr397) in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Applications WB, IF/ICC, ELISA Application Description
Tested Reactivity human
Immunogen Peptide 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name PTK2 protein tyrosine kinase 2
Synonyms FAK, PTK2, Focal adhesion kinase 1, FAK1, FADK 1
Calculated Molecular Weight 1052 aa, 119 kDa
Observed Molecular Weight 110 kDa
GenBank Accession NumberBC028733
Gene Symbol FAK
Gene ID (NCBI) 5747
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDQ05397
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

FAK (Focal adhesion kinase 1) is also named as FAK1, FADK, pp125FAK, FAK and belongs to the protein kinase superfamily. It is a critical tyrosine kinase that modulates cell adhesion, migration, proliferation and survival in response to extracellular signals (PMID:19664602). It also acts as a pivotal signal 'integrator', controlling and coordinating cellular responses that include cell migration, survival, proliferation and, epithelial tissue repair after DNA damage (PMID:20966971). This protein has some isoforms produced by alternative promoter usage and alternative splicing, and the range of the molecular weights are 100-120 kDa and 40-60 kDa.