验证数据展示
经过测试的应用
| Positive WB detected in | pervanadate treated HeLa cells |
| Positive IF/ICC detected in | A549 cells |
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:125-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 1 publications below |
产品信息
83933-1-RR targets Phospho-FAK (Tyr397) in WB, IF/ICC, ELISA applications and shows reactivity with human samples.
| Tested Applications | WB, IF/ICC, ELISA Application Description |
| Cited Applications | WB |
| Tested Reactivity | human |
| Cited Reactivity | human |
| Immunogen | Peptide 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Full Name | PTK2 protein tyrosine kinase 2 |
| Synonyms | p FAK, p-FAK, p-FAK (Tyr397), p-FAK Tyr397, p-FAK-Tyr397 |
| Calculated Molecular Weight | 1052 aa, 119 kDa |
| Observed Molecular Weight | 110 kDa |
| GenBank Accession Number | BC028733 |
| Gene Symbol | FAK |
| Gene ID (NCBI) | 5747 |
| RRID | AB_3671514 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purfication |
| UNIPROT ID | Q05397 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
FAK (Focal adhesion kinase 1) is also named as FAK1, FADK, pp125FAK, FAK and belongs to the protein kinase superfamily. It is a critical tyrosine kinase that modulates cell adhesion, migration, proliferation and survival in response to extracellular signals (PMID:19664602). It also acts as a pivotal signal 'integrator', controlling and coordinating cellular responses that include cell migration, survival, proliferation and, epithelial tissue repair after DNA damage (PMID:20966971). This protein has some isoforms produced by alternative promoter usage and alternative splicing, and the range of the molecular weights are 100-120 kDa and 40-60 kDa.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for Phospho-FAK (Tyr397) antibody 83933-1-RR | Download protocol |
| IF protocol for Phospho-FAK (Tyr397) antibody 83933-1-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
