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验证数据展示

  • IF Staining of A549 using CL488-83933

    IF Staining of A549 using CL488-83933

    Immunofluorescent analysis of (4% PFA) fixed A549 cells using CoraLite® Plus 488 Phospho-FAK (Tyr397) antibody (CL488-83933, Clone: 240630F8 ) at dilution of 1:200, CL594-Phalloidin (red).

CoraLite® Plus 488-conjugated Phospho-FAK (Tyr397) Recombinant antibody

Phospho-FAK (Tyr397) Uni-rAbTM Recombinant Antibody for IF/ICC
Cat No. CL488-83933

产品说明书

CloneNo. 240630F8

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

IF/ICC

别名

FAK (Tyr397), p FAK, p FAK (Tyr397), p FAK Tyr397, p-FAK

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  {{ptg:cur_Conjugation}}
规格: 

-/ -

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经过测试的应用

Positive IF/ICC detected inA549 cells
Planning an IHC experiment? We recommend our IHCeasy FAK Ready-To-Use IHC Kit. FAK primary antibody included.

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-83933 targets Phospho-FAK (Tyr397) in IF/ICC applications and shows reactivity with human samples.

经测试应用 IF/ICC Application Description
经测试反应性 human
免疫原 Peptide 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 PTK2 protein tyrosine kinase 2
别名 FAK (Tyr397), p FAK, p FAK (Tyr397), p FAK Tyr397, p-FAK
计算分子量 1052 aa, 119 kDa
观测分子量 110 kDa
GenBank蛋白编号BC028733
基因名称 FAK
Gene ID (NCBI) 5747
RRIDAB_3673326
偶联类型 CoraLite® Plus 488 Fluorescent Dye
最大激发/发射波长493 nm / 522 nm
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ05397
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

FAK (Focal adhesion kinase 1) is also named as FAK1, FADK, pp125FAK, FAK and belongs to the protein kinase superfamily. It is a critical tyrosine kinase that modulates cell adhesion, migration, proliferation and survival in response to extracellular signals (PMID:19664602). It also acts as a pivotal signal 'integrator', controlling and coordinating cellular responses that include cell migration, survival, proliferation and, epithelial tissue repair after DNA damage (PMID:20966971). This protein has some isoforms produced by alternative promoter usage and alternative splicing, and the range of the molecular weights are 100-120 kDa and 40-60 kDa.

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 Phospho-FAK (Tyr397) antibody CL488-83933Download protocol
Standard Protocols
Click here to view our Standard Protocols