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  • Featured Product
  • KD/KO Validated

CoraLite®555-conjugated FAK Monoclonal antibody

FAK Monoclonal Antibody for IF/ICC

Host / Isotype

Mouse / IgG2c

Reactivity

human, mouse, rat

Applications

IF/ICC

Conjugate

CoraLite®555 Fluorescent Dye

CloneNo.

2C5B9

Cat no : CL555-66258

Print datasheet

Synonyms

FADK, FADK 1, FAK, FAK1, Focal adhesion kinase 1, pp125FAK, Protein tyrosine kinase 2, PTK2, PTK2 protein tyrosine kinase 2

验证数据展示

  • IF Staining of A549 using CL555-66258

    IF Staining of A549 using CL555-66258

    Immunofluorescent analysis of (-20°C Methanol) fixed A549 cells using CoraLite®555 FAK antibody (CL555-66258, Clone: 2C5B9 ) at dilution of 1:200.

  • IF Staining of HUVEC using CL555-66258

    IF Staining of HUVEC using CL555-66258

    Immunofluorescent analysis of (4% PFA) fixed HUVEC cells using CoraLite®555 FAK antibody (CL555-66258, Clone: 2C5B9 ) at dilution of 1:200, CL488-Phalloidin (green).

  • IF Staining of A549 using CL555-66258

    IF Staining of A549 using CL555-66258

    Immunofluorescent analysis of (-20°C Methanol) fixed A549 cells using CoraLite®555 FAK antibody (CL555-66258, Clone: 2C5B9 ) at dilution of 1:200, CoraLite®488 Beta Actin antibody (CL488-66009, Clone: 2D4H5, green).

经过测试的应用

Positive IF/ICC detected inA549 cells, HUVEC cells
Planning an IHC experiment? We recommend our IHCeasy FAK Ready-To-Use IHC Kit. FAK primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 66258-1-Ig

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL555-66258 targets FAK in IF/ICC applications and shows reactivity with human, mouse, rat samples.

Tested Applications IF/ICC Application Description
Tested Reactivity human, mouse, rat
Immunogen FAK fusion protein Ag17966 种属同源性预测
Host / Isotype Mouse / IgG2c
Class Monoclonal
Type Antibody
Full Name PTK2 protein tyrosine kinase 2
Synonyms FADK, FADK 1, FAK, FAK1, Focal adhesion kinase 1, pp125FAK, Protein tyrosine kinase 2, PTK2, PTK2 protein tyrosine kinase 2
Calculated Molecular Weight 1052 aa, 119 kDa
Observed Molecular Weight 110 kDa
GenBank Accession NumberBC028733
Gene Symbol FAK
Gene ID (NCBI) 5747
RRIDAB_2919682
Conjugate CoraLite®555 Fluorescent Dye
Excitation/Emission Maxima Wavelengths557 nm / 570nm
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ05397
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

FAK (Focal adhesion kinase 1) is also named as FAK1, FADK, pp125FAK, FAK and belongs to the protein kinase superfamily. It is a critical tyrosine kinase that modulates cell adhesion, migration, proliferation and survival in response to extracellular signals (PMID:19664602). It also acts as a pivotal signal 'integrator', controlling and coordinating cellular responses that include cell migration, survival, proliferation and, epithelial tissue repair after DNA damage (PMID:20966971). This protein has some isoforms produced by alternative promoter usage and alternative splicing, and the range of the molecular weights are 100-120kD and 40-60kD.

实验方案

Product Specific Protocols
IF protocol for CL555 FAK antibody CL555-66258Download protocol
Standard Protocols
Click here to view our Standard Protocols