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FAPA Recombinant antibody, PBS Only

FAPA Uni-rAbTM Recombinant Antibody for WB, FC, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, FC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

241129B4

Cat no : 84018-4-PBS

Print datasheet

Synonyms

FAP, EC:3.4.21.-, EC:3.4.14.5, Dipeptidyl peptidase FAP, Antiplasmin-cleaving enzyme FAP, soluble form

验证数据展示

  • WB analysis of U-87 MG using 84018-4-RR (same clone as 84018-4-PBS)

    WB analysis of U-87 MG using 84018-4-RR (same clone as 84018-4-PBS)

    U-87 MG cells were subjected to SDS PAGE followed by western blot with 84018-4-RR (FAP antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84018-4-PBS in a different storage buffer formulation.

  • FC experiment of Transfected HEK-293T using 84018-4-RR (same clone as 84018-4-PBS)

    FC experiment of Transfected HEK-293T using 84018-4-RR (same clone as 84018-4-PBS)

    1x10^6 HEK-293T cells or Transfected HEK-293T cells were surface stained with 0.25 ug FAP Recombinant antibody (84018-4-RR, Clone:241129B4) and Multi-rAb CoraLite ® Plus 647-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR005). Cells were not fixed. This data was developed using the same antibody clone with 84018-4-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 84018-4-RR

    Affinity and Kinetic Characterization of 84018-4-RR

    Biolayer interferometry (BLl) kinetic assays of 84018-4-RR against Human FAP were performed. The affinity constant is 0.126 nM.

Planning an IHC experiment? We recommend our IHCeasy FAP Ready-To-Use IHC Kit. FAP primary antibody included.

产品信息

84018-4-PBS targets FAPA in WB, FC, Indirect ELISA applications and shows reactivity with human samples.

Tested Applications WB, FC, Indirect ELISA Application Description
Tested Reactivity human
Immunogen Recombinant protein 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name fibroblast activation protein, alpha
Synonyms FAP, EC:3.4.21.-, EC:3.4.14.5, Dipeptidyl peptidase FAP, Antiplasmin-cleaving enzyme FAP, soluble form
Calculated Molecular Weight88 kDa
Observed Molecular Weight 80 kDa
GenBank Accession NumberNM_004460.4
Gene Symbol FAP
Gene ID (NCBI) 2191
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDQ12884-1
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Fibroblast activation protein-alpha (FAP-alpha) is a cell surface glycoprotein serine protease, which can participate in extracellular matrix degradation and is involved in many cellular processes including tissue remodeling, fibrosis, wound healing, inflammation and tumor growth (PMID: 14751930; 16223769). FAP-alpha is a single transmembrane protein that locates the cytoplasm and plasma membrane. Immunohistochemical studies have shown that FAP-alpha is transiently expressed in certain normal fetal mesenchymal tissues but that normal adult tissues as well as malignant epithelial, neural, and hematopoietic cells are generally FAP-alpha-negative. However, most of the common types of epithelial cancers contain abundant FAP-alpha-reactive stromal fibroblasts (PMID: 7911242).